祛痹方对胶原诱导性关节炎大鼠滑膜血管生成调控因子的影响

Effect of Qubi Recipe on the vascular generate regulatory factors in synovium of collagen-induced arthritis rats

目的:从滑膜新生血管生成的角度,部分揭示祛痹方治疗类风湿关节炎(RA)的作用机制。方法:选用雄性SD大鼠,随机选取12只作为空白对照组,其余60只采用尾根部皮下注射Ⅱ型胶原与不完全弗氏佐剂的方法制作胶原诱导性关节炎(CIA)模型。15d后将CIA大鼠随机分为模型组、雷公藤多苷组、祛痹方高、低剂量组,随即灌胃给药,1次/d,连续4周。采用免疫组化的方法检测大鼠膝关节滑膜组织中fins样酪氨酸激酶(FLT-4)和胎肝激酶受体(FLK-1)的变化。结果:与空白对照组比较,模型组大鼠FLK-1、FLT-4明显升高(P<0.01);雷公藤多苷组和祛痹方高剂量组FLK-1、FLT-4水平较模型组明显降低(P<0.01),且祛痹方高剂量组FLK-1较雷公藤多苷组明显降低(P<0.05)。结论:祛痹方治疗RA的功效与抑制新生血管生成相关。

Objective： From the perspective of synovial angiogenesis, to investigate the mechanism of Qubi Recipe in the treatment of rheumatoid arthritis（RA）. Methods： Twelve male SD rats were randomly selected as blank control group, and 60 male SD rats were established as experiment arthritis rat model by subcutaneous injection of Ⅱ collagen and non-complete Freunds adjuvant. After 15 days, 60 CIA rats were randomly divided into model group, tripterygium glycosides group, Qubi Recipe high and low-dose groups. The rats in the treatment groups were administered intragastrically every day, for 4 weeks. The changes offins-like tyrosine kinase（FLT-4） and fetal liver kinase receptor（FLK-1） on synovial tissues in knee were detected by immunohistochemistry method. Results： Compared with the blank control group, the expression of FLK-1 and FLT-4 were obviously increased（P〈0.01）. The level of FLK-1 and FLT-4 were decreased in tripterygium glycosides group and Qubi Recipe high-dose group（P〈0.01）. Moreover, the expression of FLK-1 in Qubi Recipe high-dose group was signii cantly lower than those in tripterygium glycosides group（P〈0.05）. Conclusion： The treatment effect of Qubi Recipe for RA is correlated with theinhibition of angiogenesis.