宁肾颗粒对IgA肾病大鼠肾保护作用及对肾组织PDGF-BB蛋白表达的影响

Influence of Ningshen Granula on the protection effect of rats with IgA nephropathy and the expression of PDGF-BB in renal tissues

目的:研究宁肾颗粒对IgA肾病模型大鼠肾脏的保护作用及对肾组织血小板源生长因子-BB(PDGFBB)蛋白表达的影响,探讨宁肾颗粒治疗IgA肾病的作用机制。方法:将32只大鼠随机分为空白组,模型组,宁肾颗粒低、高剂量(12、24g/kg)组。采用牛血清白蛋白+LPS+CCl4方法建立实验性IgA肾病大鼠模型。造模成功后,每天分早、晚各给药或等体积0.9%氯化钠溶液1次,连续4周。干预后,检测尿液红细胞数、24h尿蛋白定量,观察肾脏病理及免疫病理,用Western blot法检测肾组织PDGF-BB蛋白表达。结果:与空白组比较,模型组大鼠尿红细胞数、24h尿蛋白定量、肾小球系膜区增殖、IgA荧光强度均增加,肾组织PDGF-BB蛋白表达上调(P<0.05,P<0.01)。与模型组比较,宁肾颗粒低、高剂量组大鼠尿红细胞、24h尿蛋白、肾小球IgA荧光强度均有降低(P<0.05,P<0.01),宁肾颗粒高剂量组肾脏系膜区增殖减轻、肾组织PDGF-BB蛋白表达降低(P<0.01)。结论:宁肾颗粒对IgA肾病模型大鼠肾脏具有保护作用,其机制可能与抑制肾脏PDGF-BB蛋白上调有关。

Objective： To research the protection effect of Ningshen Granula on kidney of rats with IgA nephropathy, and the inl uence on the expression of platelet derived growth factor-BB（PDGF-BB）, and to investigate the therapeutic mechanism of Ningshen Granula for IgA nephropathy. Methods： Thirty-two rats were randomly divided into control group, model group, Ningshen Granula low-dose（12g/kg） and high-dose groups（24g/kg）. The IgA nephropathy rat model was established according to the method of intragastric administration with lipopolysaccharide, bovine serum albumin and carbon tetrachloride. After the models were established, the rats of the four groups were administered in the morning and evening on every day for consecutive 4 weeks. The red blood cell in urine, 24 hours protein quantitative, renal pathology structure and immunopathogenesis were tested. The Western blot was used to test the expression of PDGF-BB. Results： Compared with the control group, the red blood cell in urine, 24 hours protein quantitative, proliferation of glomerular mesangial area and intensity of glomerular IgA immunol uorescence of the rats in the model group increased signii cantly, and the expression of PDGF-BB of renal tissue increased（P〈0.05, P〈0.01）. Compared with the model group, the red blood cell in urine, 24 hours protein quantitative and intensity of glomerular IgA immunol uorescence of the rats in Ningshen Granula low-dose and high-dose groups were decreased（P〈0.05, P〈0.01）. Compared with the model group, proliferation of glomerular mesangial area and expression of PDGF-BB protein of renal tissue in Ningshen Granula high-dose group were decreased signii cantly（P〈0.01）. Conclusion： Ningshen Granula could protect the kidney of IgAN rats, and the mechanism might be related to the inhibition of the up-regulation expression of PDGF-BB in renal.