β2-糖蛋白Ⅰ与抗心磷脂抗体阳性先兆流产的关系以及安子合剂干预作用的实验研究

Research on Relationship between β2-GPⅠand ACA-Positive Threatened Abortion and Intervention Effects of Anzi Mixture

目的阐述β2-糖蛋白Ⅰ（β2-GPⅠ）在抗心磷脂抗体阳性先兆流产中诱导抗体产生的机制,为安子合剂治疗ACA阳性先兆流产的进一步研究打下良好的实验基础。方法以β2-GPⅠ为免疫原,构建流产小鼠模型;采用酶联免疫吸附法（ELISA）检测抗心磷脂抗体阳性先兆流产模型小鼠血清中ACA、抗β2-GPⅠ抗体。结果①成功复制模型：胚胎丢失率模型组明显高于空白组（P〈0.05）,模型组抗β2-GPⅠ抗体浓度、ACA滴度均显著高于空白组（P〈0.05）;②安子合剂低剂量组能明显降低抗β2-GPⅠ抗体浓度和ACA滴度（P〈0.01）,且效果优于安子合剂中剂量组、高剂量组以及阿司匹林组（P〈0.05～0.01）。结论①抗β2-GPⅠ抗体、ACA均是导致免疫性复发流产的免疫因素;②安子合剂通过降低ACA滴度和抗β2-GPⅠ抗体浓度改善ACA阳性先兆流产妊娠结局;③安子合剂低剂量组具有临床等效性,随着剂量的增加,反而引起代谢障碍而使得效果逐渐减弱。

OBJECTIVE To investigate the mechanism ofβ2-GPⅠin ACA-positive threatened abortion,and to lay agood experimental foundation for the further study of treatment of Anzi mixture in ACA-positive threatened abortion.METHODSβ2-GPⅠ,as an immunogen,was applied to establish the abortion model;the ACA andβ2-GPⅠantibody in serum of abortion model rats were detected by enzyme linked immunosorbent assay（ELISA）.RESULTS ①Successful established animal model：The embryo loss rate of model group was obviously higher than that of the blank group.The concentration ofβ2-GPⅠantibody and drop degree of ACA of model group was obviously higher than that of blank group;②The low dose group obviously decreased the concentration ofβ2-GPⅠantibody and the drop degree of ACA,which of the effect was better than the medium dose group,the high dose group and the aspirin group.CONCLUSION ①β2-GPⅠantibody and ACA are immune factors which lead to immunity recurrent miscarriage;②Anzi mixture may improve pregnancy outcome of ACA-positive threatened abortion by reducing the drop degree of ACA andβ2-GPⅠantibody concentration;③The low dose group has clinical equivalence,while the increasing of dose may lead to metabolic disorders and reduce curative effect.