摘要
本文克隆了全基因3 906bp的热脂环酸杆菌的耐热酸性α-淀粉酶,此酶表达的蛋白分子量为140KD,有1 301个氨基酸。将此基因重组克隆到家蚕杆状病毒表达转移载体pVL1393中,使用一般方法获得重组病毒,并将该病毒感染家蚕,则酸性α-淀粉酶在家蚕中正常表达,对表达产物进行检测,此表达酶有淀粉酶活性。
The acid-stable alpha-amylase which was cloned from the Alicylobacillus acidocaldarius expressed by Bombyx mori bioreactor was taken to do the experiment in this study .The nucleotide sequence of Open Reading Frame of the gene acid-stableα-amylase was 3 906 bp ,encoding a polypeptide of 1 301 amino acids .The calculated molecular weight of this protein AMY was about 140KD .In this study ,recombinant virus was injected into Bom-byx mori using general method .The result is that AMY was expressed in silkworm larva .And the detection of the expression product showed amylase activity .
出处
《北方蚕业》
2014年第2期10-12,共3页
North Sericulture
关键词
Α-淀粉酶
重组病毒
克隆表达
α-amylase
recombinant virus
clone and expression
