miR-145、miR-21、miR-10b在乳腺癌中的表达及意义

miR-145, miR-21, miR-10b expression and significance in breast cancer

目的 探讨miR-145、miR-21、miR-10b在乳腺癌中的表达及意义,为进一步提高乳腺癌诊治水平提供理论实践基础.方法 采用Real-time PCR法检测35例乳腺癌组织及其癌旁正常乳腺组织中miR-145、miR-21、miR-10b的表达,分析其与乳腺癌临床病理特征的关系;同时用免疫组化检查乳腺癌中ER、PR的表达;荧光原位杂交检测HER-2、TOP 2A的表达.结果 miR-145在乳腺癌组织中的表达低于癌旁正常组织,仅为正常组织的0.78倍,但差异无统计学意义,在肿块大小、淋巴结转移、ER阳性、HER-2阳性、TOP 2A阳性患者中低表达（P＜0.05）,与年龄、月经、PR表达无关.miR-21、miR-10b在乳腺癌组织中的表达明显高于癌旁组织,分别是正常组织的8.58倍、1.70倍（P＜0.05）.miR-21在年老、绝经、淋巴结转移、HER-2阳性患者中高表达（P＜0.05）,与肿块大小、ER、PR、及TOP 2A的表达程度无关.miR-10b在肿块大小、淋巴结转移、TOP 2A阳性中高表达（P＜0.05）,而与患者年龄、月经、ER、PR、HER-2等无关.结论 miR-145、miR-21、miR-10b在乳腺癌发展、侵袭的过程中可能发挥重要作用.

Objective To investigate the significance of miR-145,and miR-21,the role of miR-10b in breast cancer,providing theoretical and practical foundation for further improving the diagnosis and treatment of breast cancer.Methods Real-time RT-PCR detected 35 cases of breast cancer and adjacent normal breast tissue miR-145,miR-21,miR-10b expression,and analysis of their relationship with clinico-pathological features of breast cancer; the expression was examined by immunohistochemistry in breast cancer ER,PR expression; fluorescencein situ hybridization in detection of HER-2,TOP 2A.Results Expression of miR-145 in breast cancer tissues was lower than in adjacent normal tissue,only 0.78 of normal tissue,but without statistical significance.miR-145 in large lumps,lymph node metastasis,ER-positive,HER-2 positive,TOP 2A-positive patients with low expression （P ＜ 0.05）,with age,menstruation,PR expression is independent.Expression of miR-21,miR-10b in breast cancer tissues was significantly higher than that in adjacent normal tissue,which are 8.58,1.70 times the normal tissues （P ＜0.05）.MiR-21 in old age,menopause,lymph node metastasis,HER-2 positive patients with high expression （P ＜0.05）,regardless of tumor size,ER,PR,and the degree of expression of TOP 2A; miR-10b in large lumps,lymph node metastasis,TOP 2A high positive expression （P ＜ 0.05）,while with age,menstruation,ER,PR,HER-2 independent.MiR-10b in large lumps,lymph node metastasis,the positive expression TOP 2A high expression （P ＜ 0.05）,and with age,unrelated to menstruation,ER,PR,HER-2 expression.Conclusions miR-145,miR-21 and miR-10b in breast cancer development,invasion process may play an important role.