腺病毒介导血红素加氧酶-1基因转染治疗大鼠脊髓缺血再灌注损伤

Adenovirus-mediated gene transfer of rat heme oxygenase-1 against rat spinal cord ischemia-reperfusion injury

目的 观察腺病毒介导血红素加氧酶-1（HO-1）基因对大鼠脊髓缺血再灌注损伤的保护作用。方法 雄性SD大鼠分成假手术组（A组）、缺血再灌注组（B组）、AdV-rHO-1处理组（C组）。A组为对照组,B、C组阻断腹主动脉30 min后,暴露大鼠脊髓分别注入AdV-EGFP、AdV-rHO-1病毒载体。各组于缺血再灌注12 h、1、2、3、7 d测定后肢神经功能评分、病理学、HO-1、HO-1 mRNA的表达和凋亡细胞。结果 C组后肢神经功能评分（2.83±0.75）在缺血再灌注2 d后明显好于B组（1.50±0.55）（P〈0.01）。C组（35.85±6.59）HO-1表达量较A、B组明显增多（P〈0.01）,C组（1.83±0.28）HO-1的mRNA表达量较A组和B组明显升高（P〈0.01）。结论 腺病毒介导的HO-1基因能有效转染缺血再灌注脊髓组织,对缺血再灌注的脊髓有保护作用。

Objective To study the protective effect of adenovirus-mediated heine oxygenase-1 （ HO-1 ） transfection on the spinal cord ischemia-repeffusion injury （IRI） in rats. Methods SD rats were randomly divided into sham operation group （group A ）, ischemia reperfusion group （group B ） and AdV-rHO-1 group （group C）, The rats in groups B and C were given spinal cord ischemia for 30 min. Physiological saline, AdV-EGFP, and AdV-rHO-1 were injected in groups A, B and C, respectively. Hind legs nerve function grading, apoptotic cells, immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR） were used to evaluate the degree of spinal cord injury. Results The hind legs nerve function grading （2. 83 ±0. 75） in group C was obviously better than in group B （1.5 ±0. 55） after reperfusion for 2 days （ P 〈 0.01 ）. The histopathologic changes in group C were obviously milder than those in group B. Immunohistochemistry and RT-PCR indicated that transgene was already expressed in group C. The level of HO-1 in group C （35. 85 ±6. 59） was higher than that in group A （7. 24 ± 1.15） or group B （ 8.21 ± 3.05 ） （ P 〈 0. 01 ）. Concbmion Adenovirus-mediated gene transfection of rHO-1 has significantly protective effect on spinal cord IRI.