NS-398联合白细胞介素-6抗体对肝门胆管癌细胞株QBC939的增殖抑制及前列腺素E2的影响

The research of NS-398 combined with interleukin-6 antibody inhibits the proliferation of hilar cholangiocarcinoma cell line QBC939 and decreases the level of prostaglandin E2

目的 观察环氧合酶-2（COX-2）选择性抑制剂NS-398联合白细胞介素-6（IL-6）抗体对人肝门胆管癌QBC939细胞的增殖抑制及降低前列腺素E2（PGE2）含量的协同作用。方法不同浓度NS-398（25、50、100μmol/L）、IL-6抗体（0.1、1.0、10.0 mg/L）及联合用药（NS-398 100μmol/L＋IL-6抗体10 mg/L）作用于QBC939细胞24、48、72 h后,细胞计数试剂盒（CCK-8）法测定细胞增殖抑制率,酶联免疫吸附试验（ELISA）法检测细胞PGE2含量。结果 不同浓度的NS-398、IL-6抗体对QBC939细胞均有抑制作用,且联合效果明显优于单药,各组与对照组比较差异均有统计学意义（P〈0.05）,ELISA分析：NS-398（100μmol/L）、IL-6抗体（10 mg/L）、联合组作用细胞72 h后每孔PGE2含量分别为（25.606±5.676）、（36.454±6.444）、（18.223±4.168）ng,与阴性对照组每孔（106.781±12.423）ng比较差异均有统计学意义（P〈0.05）。结论NS-398和IL-6抗体均有抑制人肝门胆管癌QBC939细胞增殖的作用,亦能降低细胞PGE2的含量,作用具有浓度、时间依赖性且联合作用更强。

Objective To examine the synergistic effect of NS-398 [ a specific cyclooxygenase-2 （COX-2） inhibitor and intedeukin-6 （IL-6） antibody on proliferation inhibition and prostaglandin E2 （ PGE2 ） levels of Hilar eholangiocareinoma （HCCA） cell line QBC939. Methods QBC939 cells were divided into 25, 50 and 100 μmol/L NS-398 groups. 0. 1, 1.0, 10. 0 mg/L IL-6 antibody groups and NS-398 100 p, mol/L ± IL-6 antibody 10 mg/L combination groups. The cells were treated for 24, 48, 72 h. The proliferation-inhibitory activity of QBC939 cells were detected by cell counting kit-8 （CCK-8）. The PGE2 levels in the supernatant of cultured cells were quantitated by ELISA. Results After treated with NS-398 and IL-6 antibody respectively or in combination, cells proliferation were obviously inhibited, and the role in combined treatment groups was more significant than monotherapy groups compared with the negative control group （P 〈0. 05）. ELISA showed that： After 72 h, the PGE2 value of NS-398 100 μmol/L group, IL-6 antibody 10 mg/L group and NS-398 100 μmol/L ± IL-6 antibody 10 mg/L group were （25. 606 ± 5. 676） ng/well, （ 36. 454 ± 6. 444 ） ng/well, （ 18. 223 ± 4. 168 ） ng/well and respectively compared with the negative control group （ 106. 781 ± 12. 423 ） ng/well （ P 〈 0. 05 ）. Conclusion NS-398 and IL-6 antibody had inhibitory effects on the growth of HCCA cell line QBC939, and they could inhibit the expressions of PGE2 in QBC939 cells in a dose and time dependent manner. And the combination inhibitory effect was more apparent.