表面等离子体共振传感技术和生物分析仪

Surface Plasmon Resonance Sensing Technology and Bioanalytical Instrument

表面等离子体共振(surface plasmon resonance,SPR)传感技术是生物化学分析领域常用的分析手段和研究工具,与其相关的研究不计其数,发展日新月异。本研究小组从事SPR传感技术研究近十年,从初期的理论研究、仿真计算、传感器设计以及全自动SPR生物分析仪开发与应用研究,到目前的传感器性能提高、应用拓展,时刻关注着该项技术的最新动态。本文系统综述了SPR传感技术和生物分析仪的原理、结构以及主要功能模块,SPR传感器的调制类型、耦合方式以及SPR成像传感器;介绍了结合局域表面等离子体共振(local surface plasmon resonance,LSPR)技术、改进金属膜系设计、优化数据处理算法等提高SPR生物分析仪性能的方法;阐述了SPR传感技术和生物分析仪的最新进展,包括SPR技术和微流控芯片、电化学技术、表面增强拉曼散射技术(SERS)的联用;列举了SPR生物分析仪在临床诊断、药物筛选、生物分子研究、食品安全和环境监测等领域的应用实例;最后,分析了SPR生物分析仪面临的主要问题以及未来的发展趋势。

Surface plasmon resonance （SPR） biosensor technology, with countless researches and attractive progress, has been commonly applied in the field of biochemical analysis. From theoretical research to design and produce of bioanalytical instrument, application development and performance improvement, our research team has been engaged in SPR study for almost l0 years and always concerned about the latest progress. This paper mainly includes ： the description of principle, components and accessories of SPR bioanalytical instrument, modulation types and coupling configurations of SPR sensor, as well as SPR imaging sensor; the methods for improving the bioanalytical instrument performance, namely, the combination with local surface plasmon resonance （LSPR） technology, metal film redesign and data processing optimization; the lasted developments, involving the technology combination between the SPR and microfluidic chip, electrochemistry, surface- enhanced raman scattering （SERS） ; the applications in clinical diagnosis, drugscreening, human biomolecular research, food safety and environmental monitoring; and the main problems and future trends of SPRbioanalytical instrument.