慢性阻塞性肺疾病患者股四头肌碳酸酐酶Ⅲ蛋白及其mRNA表达

Carbonic anhydrase Ⅲ and mRNA expression levels in quadriceps femoris muscle of chronic obstructive pulmonary disease patients

目的 观察慢性阻塞性肺疾病（COPD）患者骨骼肌碳酸酐酶Ⅲ（CAⅢ）蛋白及mRNA表达水平.方法 收集骨科手术诊疗患者的股四头肌标本,对照组11例,COPD者26例;根据患者BMI、去脂肪指数,COPD者分为COPD骨骼肌萎缩组（12例）和COPD骨骼肌非萎缩组（14例）.Western blot检测3组受试者股四头肌CAⅢ蛋白表达,荧光定量PCR检测CAⅢmRNA表达水平.结果 （1）CAⅢ蛋白相对表达量对照组为1.000±0.062; COPD骨骼肌非萎缩组为1.110 ±0.014,高于对照组（P=0.0006）;COPD骨骼肌萎缩组为1.260±0.068,高于COPD骨骼肌非萎缩组、对照组（P值均=0.000 1）;3组比较差异有统计学意义（F=10.6,P＜0.05）.（2）CAⅢmRNA相对表达量对照组为1.000 ±0.115;COPD骨骼肌非萎缩组为2.170±0.412;COPD骨骼肌萎缩组为3.770±0.788,高于COPD骨骼肌非萎缩组、对照组（P值均=0.000 1）;3组比较差异有统计学意义（F=8.98,P＜0.05）.（3）COPD骨骼肌萎缩组、COPD骨骼肌非萎缩组CAⅢ蛋白相对表达量与CAⅢmRNA相对表达量有相关性（r=0.620 8,P=0.003 4;r=0.569 0,P=0.005 4）.结论 COPD者下肢骨骼肌存在CAⅢ蛋白、CAⅢmRNA表达升高,其升高的内在机制值得深入探讨.

Objective To explore the expression status of carbonic anhydrase Ⅲ （CA Ⅲ） from quadriceps femoris muscle in two kinds of muscle clinical phenotype （skeletal muscle atrophy group and skeletal muscles non-atrophy group） of chronic obstructive pulmonary disease （COPD）.Methods Totally 37 inpatients from our hospital,were divided into 11 patients without COPD and 26 patients with COPD,in addition,according to body mass index,fat free mass index and quadriceps cross-section diameter,patients with COPD were divided into 14 skeletal muscles non-atrophy patients （SMNA）and 12 skeletal muscle atrophy patients （SMA）.CA Ⅲ concentration of femoris quadriceps specimens was quantitatively determined using Western blot methods,CA Ⅲ mRNA expression levels of femoris quadriceps specimens were also quantitatively measured using RT-PCR,then compared among the 3 groups.Results There was significant difference in CA Ⅲ quantitative concentration and CA Ⅲ mRNA expression level in each group （P 〈 0.05）,further more,CA Ⅲ concentration expression level was significantly higher （P 〈 0.01）in SMA group （1.260 ± 0.068） than in SMNA group （1.110 ± 0.014）,the latter was significantly higher （P 〈 0.01） than in the control group （1.000 ± O.062）.CA Ⅲ mRNA expression level was significantly higher（P 〈 0.01） in SMNA group（2.170 ±0.412） than in the control group（1.000 ±0.115）,and was significantly lower than in SMA group （3.770 ± 0.788 ; P 〈 O.01）.CA Ⅲ concentration and CA Ⅲ mRNA expression level increased at equal pace in SMNA group and SMA group,however,CA Ⅲ quantitative concentration and CA Ⅲ mRNA expression level were inconsistent in the two groups.Conclusion The expression status of CA Ⅲ in quadriceps femoris muscle was different in two kinds of muscle clinical phenotype of COPD.