MMP-14和COX-2蛋白在胃癌侵袭转移过程中的表达和关系

Expression of MMP-14 and COX-2 in gastric cancer

目的:探讨基质金属蛋白酶-14(matrix mettalloproteinase 14,MMP-14)和环氧合酶2(cyclooxygenase 2,COX-2)蛋白在胃癌侵袭转移过程中的表达和相互关系,从而进一步揭示胃癌的发病机制.方法:采用免疫组织化学法检测人胃癌组织和正常胃组织中MMP-14和COX-2的蛋白表达情况,进一步分析二者与胃癌临床病理参数的关系,并探讨MMP-14在胃癌组织不同TNM分期中的表达特点.结果:胃癌组织中MMP-14和COX-2的阳性表达率明显高于正常胃组织(P<0.01);MMP-14的表达与胃癌分化程度、区域淋巴结转移程度、浸润深度、TNM分期相关(P<0.01),COX-2的表达与浸润深度、TNM分期相关(P<0.05),胃癌组织中MMP-14与COX-2蛋白表达呈正相关(P<0.01);随着TNM分期的增加,MMP-14表达增加且有从胞浆向胞膜转位的趋势.结论:COX-2蛋白可能通过诱导MMP-14蛋白表达上调及胞内转位,从而增加胃癌细胞的侵袭及转移能力.

AIM： To investigate the protein expression of MMP-14 and COX-2 in gastric cancer to further reveal the pathogenesis of gastric cancer.METHODS： The protein expression of MMP-14 and COX-2 was examined by immunohistochemistry in human gastric cancer tissues and normal gastric tissues, and the relationship between the protein expression and clinicopathological features was analyzed. The protein expression of MMP-14 in human gastric cancer tissues at different TNM stages was also explored.RESULTS： The expression rates of MMP-14 and COX-2 in gastric cancer tissues were significantly higher than those in normal tissues（P ＆lt; 0.01）. The expression of MMP-14 was significantly correlated with the degree of differentiation, lymph node metastasis, invasion depth and TNM stage of gastric cancer（P ＆lt; 0.01）. The expression of COX-2 was correlated with invasion depth and TNM stage of gastric cancer（P ＆lt; 0.05）. MMP-14 expression was positively correlated with COX-2 expression in gastric cancer tissues（P ＆lt; 0.01）. MMP-14 expression increased with the elevation of TNM stage and showed a tendency from the cytoplasm to cell membrane.CONCLUSION： Stimulating overexpression and intracellular translocation of MMP-14 may be one of mechanisms for COX-2 protein to contribute to the invasion and metastasis of human gastric cancer.