榄香烯乳对人胰腺癌移植瘤组织中凋亡相关蛋白表达的影响

Effect of treatment with elemene on expression of apoptosis-related proteins in pancreatic carcinoma xenografts in nude mice

目的:了解榄香烯乳干预治疗后人胰腺癌裸鼠移植瘤组织中PTEN、P53、Bcl-2蛋白的表达情况,初步探讨榄香烯乳对胰腺癌凋亡的影响.方法:构建人胰腺癌裸鼠移植瘤模型,随机分为阴性对照组、榄香烯乳小剂量组、榄香烯乳大剂量组、吉西他滨阳性对照组和联合用药组五组,分别干预处理;采用Western blot和免疫组织化学的方法检测榄香烯乳干预治疗后人胰腺癌移植瘤组织细胞中PTEN、P53、Bcl-2蛋白表达情况.结果:Western blot结果显示榄香烯乳干预治疗后人胰腺癌移植瘤细胞中PTEN和P53蛋白表达均有升高趋势,而Bcl-2蛋白表达有下降的趋势.榄香烯乳大剂量组与阴性对照组相比,P53蛋白表达显著升高(P<0.01)而Bcl-2蛋白表达显著降低(P<0.01).联合用药组与吉西他滨阳性对照组相比,P53蛋白表达显著升高(P<0.01),而Bcl-2蛋白表达降低不明显(P=0.985).免疫组织化学结果显示与Western blot结果大致相同的变化趋势.结论:一定剂量的榄香烯乳可以增强胰腺癌裸鼠移植瘤组织中P53蛋白的表达而降低Bcl-2蛋白的表达,从而起到促进胰腺癌细胞凋亡的作用.

AIM： To investigate the expression of PTEN, p53 and Bcl-2 proteins in pancreatic carcinoma xenografts in nude mice after treatment with elemene, and to explore the effect of elemene on the apoptosis of pancreatic cancer cells.METHODS： Thirty mice with subcutaneous xe- nografts of a pancreatic cancer cell line （SW1990） were randomly divided into five groups： a negative control group, low- and high-dose elemene-treated groups, a gemcitabine-treated group, and an elemene plus gemcitabine group. Western blot and immunohistochemical staining were used to determine the expression of PTEN, p53 and Bcl-2 proteins in pancreatic carcinoma xenografts in nude mice after treatment with el- emene. RESULTS： The expression of PTEN and p53 proteins had a trend of up-regulation, while the expression of Bcl-2 protein had a trend of down- regulation in pancreatic carcinoma xenografts in nude mice after treatment with elemene. The re- sults of immunohistochemical staining showed the same trend. Compared with the negative control group, the expression of p53 was significantly up-regulated, while Bcl-2 was down- regulated in the highdose elemene group. Compared with the gemcitabine-treated group, the expression of p53 was significantly upregulated, but Bcl-2 had no significant change in the combination treatment group. Immunohistochemical staining showed similar results to Western blot analysis. CONCLUSION： Elemene can upregulate the expression of PTEN and p53 proteins and down- regulate the expression of Bcl-2 protein in pancreatic cancer in a dose dependent manner, thereby inducing the apoptosis of pancreatic cancer cells.