纤维素酶在木质纤维素底物上的吸附、脱附以及循环利用

Cellulase Adsorption-desorption Behavior onto/from Lignocellulosic Substrates and Potential Recycling

纤维素酶在木质纤维素底物上的吸附是一个复杂的物理化学过程,与纤维素酶和木质纤维素底物本身的性质密切相关,而且还受水解体系的各种参数,如缓冲溶液的浓度、pH、离子强度、温度、是否添加表面活性剂等影响。酶解结束时,大量的纤维素酶吸附在残余底物上。回收吸附在残余底物上的纤维素酶,是降低生物乙醇生产中纤维素酶成本的重要策略。缓冲溶液的pH、温度、是否添加表面活性剂等也对纤维素酶的脱附有着重要的影响。本文综述了影响纤维素酶吸附、脱附的各种参数;总结了纤维素酶脱附领域的研究进展以及纤维素酶循环利用的基本流程。

Adsorption of cellulase onto lignocellulosic substrates is a complexes physical and chemical process. It was not only closely related to cellulase and substrate self, but also related to the parameters during enzymatic hydrolysis, such as buffer concentration, pH, ionic strength, temperature and surfactants. A large part of cellulase was still attached to residual substrate at the end of saccharification. Recycling of adsorbed cellulase on residual substrate could be one of the most promising strategies for cost reduction. Factors which may affect cellulase adsorption could also play important roles on eellulase desorption. This article reviews different parameters that affect cellulase adsorption and desorption and also summarizes state of the art technology for enzyme recycling. This review also gives a schematic diagram for cellulace recycling.