人参皂苷Re拮抗肠缺血/再灌注致肺损伤机制中抗炎抗氧化作用研究

Anti-inflammatory and antioxidant effect of ginsenoside Re on the lung injury induced by intestinal ischemia reperfusion in rats

目的建立大鼠肠缺血/再灌注（Ⅱ/R）致肺损伤模型,观察人参皂苷Re的抗炎抗氧化作用。方法 32只SPF级雄性SD大鼠（240∽260 g）随机分为4组（n=8）,假手术组（S组）、肠缺血/再灌注组（Ⅱ/R组,即M组）、Ⅱ/R＋人参皂苷Re高剂量组（Re-H组）、Ⅱ/R＋人参皂苷Re低剂量组（Re-L组）。建立肠缺血1 h再灌注2 h损伤模型。大鼠再灌注末取腹主动脉血,检测血清中TNF-α、IL-6、IL-10、SOD和MDA水平;取肺组织标本进行病理学检查和湿/干比测定。结果与S组相比,M组肺组织湿/干比明显增加（P〈0.01）,肺脏损伤明显,血清中IL-6、TNF-α、IL-10和MDA含量显著升高,同时SOD活力下降（P〈0.05或P〈0.01）;与M组比较,Re-H组和Re-L组可显著改善上述变化（P〈0.05或P〈0.01）,肺组织损伤程度减轻。结论人参皂苷Re预处理可通过提高机体抗氧化、抗炎能力对肠缺血/再灌注所致肺损伤起到保护作用。

Objective To study the anti-inflammatory and antioxidant effect of ginsenoside Re from Panax notoginseng（Burk.） F. H. Chen on the lung injury induced by intestinal ischemia/reperfusion（ Ⅱ /R） in rats. Methods Thirty-two Sprague-Dawley male rats were randomly divided into 4 groups： a sham group, a Ⅱ /R model group, a Re-H group（ Ⅱ /R ＋ ginsenoside Re high-dose） and a Re-L group（ Ⅱ /R ＋ ginsenoside Re low-dose）. A rat model of intestinal ischemia/reperfusion injury was seted up by 1 h of superior mesenteric artery occlusion followed by 2 h min of reperfusion. The serum samples were collected to measure the contents of TNF-α, IL-6, IL-10, MDA and SOD, while the lung tissues were harvested for morphological examination. Results Compared with the sham group, the lung tissue injury was significantly aggravated and the wet/dry ratio of lung tissue increased in M group（P 0.01）, and the levels of TNF-α, IL-6, IL-10 and MDA in the serum were significantly increased in M group（P 0.05 or P 0.01）, The activity of SOD was decreased markedly（P 0.01）. When pretreated with ginsenoside Re, the changes of TNF-α, IL-6, IL-10, SOD and MDA levels were diminished markedly（P 0.05 or P 0.01）, and the lung injury was markedly reduced. Conclusion Ginsenoside Re pretreatment can ameliorate the lung injury by improving the anti-oxidative and antiinflammatory abilities.