艾司洛尔与急性心肌梗死患者红细胞携氧能力的关系研究

Study on the Relationship Between Esmolol and Erythrocyte Oxygen Carrying Capacity in Patients with Acute Myocardial Infarction

【目的】探讨艾司洛尔是否影响急性心肌梗死患者红细胞血红蛋白的携氧能力。【方法】采集急性心肌梗死患者红细胞，悬浮于不同pH值（pH7．4、pH5．0）等渗PBS缓冲液，分别用肾上腺素、艾司洛尔、艾司洛尔＋肾上腺素预处理红细胞，在拉曼光谱点扫描检测单个活态红细胞内血红蛋白特征光谱变化。【结果】在pH5．0时，氧化血红蛋白特征峰峰强在PBS、艾司洛尔的作用下明显降低；在肾上腺素的作用下显著升高（P〈0．05）；而经艾司洛尔＋肾上腺索预处理后，肾上腺素的这一作用不明显。【结论】艾司洛尔能减轻肾上腺素在酸性环境中增加红细胞血红蛋白的氧合力的作用，增加氧从血红蛋白的解离，有利于缺氧组织的供氧。

[Objective]To explore whether esmolol affects erythrocyte oxygen carrying capacity in patients with acute myocardial infarction. [Methods]Erythrocytes from patients with acute myocardial infarction were collected and suspended in isotonic PBS buffer solution with different pH value（pH7.4 and pH5. 0）. Erythrocytes were pretreated with adrenaline, esmolol and adrenaline plus esmolol, respectively. The characteristic spectral changes of hemoglobin in single living erythrocytes were detected by using Raman spectroscopy analysis. [Results]Characteristic peak of oxyhemoglobin treated with PBS and esmolol at pH5.0 was decreased obviously, but that treated with adrenaline was increased significantly. After pretreatment with esmolol plus adrenaline, the effect of adrenaline was not obvious. [Conclusion]Esmolol can reduce the effect of adrenaline for enhancing erythrocyte oxygen carrying capacity of hemoglobin in acid environment, and increase the dissociation of oxygen from hemoglobin, and is helpful for oxygen supply of anoxia tissue.