摘要
目的研究并优化荷叶生物碱的提取工艺。方法采用酸提碱沉提取结合有机溶剂萃取纯化的方法从荷叶中提取生物碱。并通过单因素试验和正交试验,研究pH值、提取温度、提取时间、固液比等条件对荷叶生物碱提取的影响,优化工艺提取条件。结果荷叶生物碱的最佳提取工艺:提取温度90℃,pH值2.5,提取时间2 h,固液比1∶15。采用此条件提取的荷叶生物碱纯度为73.4%。提取温度、pH值对提取率影响显著。结论该荷叶生物碱的提取纯化工艺简单,成本低,提取效率高。
Objective To optimize the extraction and purification technology of alkaloid from lotus leaf . Methods Alkaloid was extracted from lotus leaf by acid extraction and alkaline deposition combined with organic solvent extraction and purification .And the extraction conditions were optimized through single factor test and or-thogonal experiment .Results The optimum condition for extraction was summarized ,namely ,extracting temp .90℃ ,pH value 2 .5 ,duration of extraction 2h and solid to liquid ratio 1：15 .Conclusion The extraction and purifi-cation technology of alkaloid was simple ,high efficiency and affordable with convenient source of materials .
出处
《湘南学院学报（医学版）》
2014年第2期20-23,共4页
Journal of Xiangnan University（Medical Sciences）
基金
郴州市科技计划项目(CZ2013092)
中南林业科技大学对口支援项目(2012XN08)
湘南学院科研课题(2013YJ45)
湘南学院2012大学生创新性实验计划项目(23)
湖南省"十二五"重点学科流行病与卫生统计学
湘南学院"十二五"重点学科免疫学及湘南学院病理研究所资助
关键词
荷叶
生物碱
提取
分离
纯化
Lotus leaf
Alkaloid
Extraction
Separation
Purification
