结直肠癌k-ras基因与多药耐药相关蛋白相关性研究

Correlation between the K-ras gene and multidrug resistance associated protein in colorectal cancer

目的:探讨结直肠癌(CRC)中K-ras基因突变和多药耐药相关蛋白的表达及两者的关系。方法:利用免疫组织化学、实时荧光定量PCR技术检测K-ras基因及多药耐药相关蛋白在CRC中的表达。结果:结直肠癌中Pgp、Topo-II、CerbB-2、GST-π和Ki-67的阳性率分别为15.8%,84.21%,26.13%,71.42%,32.67%。Pgp、Topo-II的表达和K-ras突变率与肿瘤发病的性别、类型、分化程度、浸润深度及有无淋巴结转移无关(P>0.05),CerbB-2、GST-π的阳性率与肿瘤淋巴结转移相关(P<0.05),Ki-67的阳性率与肿瘤浸润深度和淋巴结转移相关(P<0.05)。K-ras基因突变率为28.94%,与CerbB-2的表达呈正相关(P<0.05)。结论:联合检测多药耐药相关蛋白及K-ras突变有助于临床判定CRC对化疗药物的敏感性、制定个性化化疗方案提供依据。

Purpose To investigate the expression of the multidrug resistance and K-ras gene mutations in the colorectal cancer and their relationship. Methods：Using Immunohistochemistry and real-time quantitative PCR technique. Results：The expression rate of Pgp , Topo-Ⅱ , CerbB-2 , GST-π and Ki-67 were 15 . 8%, 84 . 21%, 26 . 13%, 71 . 42%, 32 . 67% in the colorectal cancer . The expression rate of Pgp , Topo-Ⅱ and K-ras mutation was not significant （P〉0 . 05） with tumor incidence of gender , type , differentiation , depth of invasion and lymph node metastasis （P〉0 . 05）, while CerbB-2 , GST-π was significant with lymph node metastasis （P〈0 . 05）, Ki-67 was significant with tumor infiltration depth and lymph node metastasis （P〈0 . 05）. K-ras gene mutation rate was 28 . 94%, and was positively correlated with CerbB-2 expression （P〈0 . 05）. Conclusion：Combined detection of multidrug resistance- associated protein and K-ras gene mutations can contribute to judgment CRC sensitivity to chemotherapeutic drugs and provide the theory to formulate personalized chemotherapy .