甘蓝硫代葡萄糖苷总量性状的QTL定位及分析

Mapping QTL and Analysis on Glucosinolates Total Content in Cabbage

采用HPLC方法分析了作图群体双亲及F283个单株的硫代葡萄糖苷总含量,在已构建的高密度甘蓝遗传图谱的基础上,运用MapQTL 4.0软件对结球甘蓝F2群体硫代葡萄糖苷总含量进行QTL定位和分析。最终检测到3个控制甘蓝硫代葡萄糖苷含量性状的QTLs(分别位于LG7上的qGS-7-1和位于LG8上的qGS-8-1、qGS-8-2),其贡献率分别为13.6%,7.5%,5.6%。并得到同时控制硫代葡萄糖苷含量QTLs共分离的分子标记me27-em25-2和E38M55-7,均可为辅助选育富含硫代葡萄糖苷甘蓝品种提供理论依据。

Glucosinolates,anticancer active substances,are widely present in Brassica crops.It will be of important significance to carry out the breeding of high content of Glucosinolates new varieties of cabbage.This study analyzed total glucosinolate content of F2 mapping population parents and that of 83 individual plants by HPLC methods.Based on the cabbage high-density genetic linkage map which had been constructed previously,QTL mapping and analysis related to Glucosinolates total content in the individuals of F2 were performed using MapQTL 4.0.Three QTLs（ qGS-7-1,qGS-8-1,qGS-8-2） located on LG7 and LG8 controlling the traits of Glucosinolates total content in cabbage were detected.The contribution rate were 13.6%,7.5% and 5.6%,respectively.Meanwhile,two molecular markers（ me27-em25-2,E38M55-7） coseparating with the QTLs could be useful for marker assisted selection of cabbage breeding rich in Glucosinolates.