摘要
为研究牦牛肌肉中脂肪酸组成特征及其形成机制,以牦牛和黄牛背最长肌为材料,利用气象色谱-质谱联用仪(GC-MS)进行脂肪酸的定性定量分析,并通过Real-time PCR技术检测FASN基因在牦牛和黄牛背最长肌中mRNA的表达水平,同时分析了其与脂肪酸组成的相关性。结果发现,牦牛背最长肌中单不饱和脂肪酸(MUFA)和多不饱和脂肪酸(PUFA)含量均极显著高于黄牛(P<0.01),而饱和脂肪酸(SFA)显著低于黄牛(P<0.05)。牦牛背最长肌中FASN基因的mRNA表达水平极显著高于黄牛(P<0.01)。牦牛FASN基因mRNA表达量只与牦牛C10:0呈极显著正相关(P<0.01),与黄牛脂肪酸组成无显著的相关性(P>0.05)。
In order to study the characteristics and mechanism of fatty acid composition in yak,the fatty acids composition and content of Longissimus dorsi muscle in yak and cattle were determined by using gas chromatographmass spectrometer-computer（ GC-MS）,while the FASN gene mRNA expression level was investigated by Real-time PCR,and the correlation analysis was carried out.The results showed that the monounsaturated fatty acid（ MUFA）and polyunsaturated fatty acids（ PUFA） in yak was extremely significantly higher than in cattle（ P〈 0.01）,while the saturated fatty acid（ SFA） was significantly lower than in cattle（ P〈 0.05）.The FASN mRNA expression in yak was extremely significantly higher than in cattle（ P〈 0.01）.There is an extremely significant positive correlation（ P〈 0.01） between the FASN mRNA expression level and content of C10： 0 in yak,no significant correlation（ P〈 0.05） was found in cattle.
出处
《华北农学报》
CSCD
北大核心
2014年第3期159-163,共5页
Acta Agriculturae Boreali-Sinica
基金
国家肉牛牦牛产业技术体系牦牛选育项目(nycytx-38)
甘肃牧区生产生态生活优化保障技术集成示范项目(2012BAD13B05)
