miRNA-451对结肠癌细胞侵袭力的影响研究

The effects of miRNA-451 on cell invasion of colorectal cancer

目的分析miRNA-451(miR-451)对结肠癌细胞SW480的影响,探讨miRNA-451对结肠癌细胞侵袭力的作用价值。方法化学合成miR-451mimics,脂质体包裹转染SW480细胞,分为miR-451转染组、无关序列转染组及对照组(未处理),荧光染料标记法检测转染效率,细胞计数试剂盒-8检测细胞增殖情况,流式分析检测细胞凋亡情况以及Transwell小室检测侵袭能力情况。结果 miRNA-451对结肠癌细胞SW480的增殖能力和凋亡情况无显著影响,但是对其侵袭力具有显著的抑制作用。结论 miR-451mimics对结肠癌细胞系SW480具有抑制作用,对结肠癌细胞侵袭力具有抑制效果,值得临床深入研究。

Objective To analyze the effects of miRNA-451 on colonic SW480 cells and explore the value of miRNA-451 on human colorectal cancer cell invasiveness.Methods We used chemical synthesis to make miR-451 mimics.SW480 cells were transfected by liposome-coated.They were divided into miR-451 transfection group,nonsequential transfection and control groups（untreated）.We used fluorescent dye marker to detect the transfection efficiency and cell counting kits-8 to detect of cell proliferation.The flow analysis was used to detect cell apoptosis and Transwell Chamber invasion.Results There was no significant impact for MiRNA-451 on the apoptosis and colon cancer cells proliferation of SW480,but there was significant invasiveness for MiRNA-451 on inhibition.Conclusion MiR-451 mimics played an inhibitory role in colon cancer cell line SW480.It has invasiveness-inhibiting effect on colon cancer cells.It is worth a deep clinical research.