血脂异常者血清MCP-1、HO-1、APN和TNF-α水平的检测及临床价值

Detection and clinical value of serum levels of monoCyse chemoattractant protein-1,heme oxygenase-1,adiponectin and tumor necrosis factor-α in patients with dyslipidemia

目的探讨血脂异常者血清单核细胞趋化蛋白-l(MCP-1)、血红素加氧酶-1(HO-1)、脂联素(APN)及肿瘤坏死因子α(TNF-α)水平的检测及临床价值。方法采用酶联免疫吸附法(ELISA)检测85例血脂异常者和35例健康对照者血清MCP-1、HO-1、APN及TNF-α的水平,同时检测血脂异常者血清其他脂类代谢指标[总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)]的水平,并分析MCP-1、HO-1、APN及TNF-α与脂类代谢指标的相关性。结果血脂异常者血清MCP-1、HO-1和APN水平较健康对照组均显著升高,差异具有统计学意义(P<0.01),而TNF-α水平较健康对照组则明显降低,差异具有统计学意义(P<0.01);同时血脂异常者血清HO-1水平与MCP-1及LDL-C水平均呈正相关关系;APN水平则与TNF-α呈负相关关系,与LDL-C水平呈正相关关系;而健康对照组的HO-1水平与MCP-1水平无明显关系。结论血脂异常者血清MCP-1、HO-1和APN水平显著升高,而TNF-α水平则明显降低,说明MCP-1、HO-1、APN及TNF-α可能参与了动脉粥样硬化的发生及发展,并可能成为动脉粥样硬化潜在的预防或治疗靶点。

Objective To investigate the clinical value of serum levels of monoCyse chemoattractant protein-1（ MCP-1）,heme oxygenase-1（ HO-1）,adiponectin（ APN） and tumor necrosis factor-α（ TNF-α） in patients with dyslipidemia. Methods The serum levels of MCP-1,HO-1,APN and TNF-α were detected by using enzyme linked immunosorbent assay（ ELISA） in 85 patients with dyslipidemia and 35 healthy controls. Meanwhile the serum levels of other lipid metabolism parameters including total cholesterol（ TC）,triglyceride（ TG）,low density lipoprotein cholesterol（ LDL-C） and high-density lipoprotein cholesterol（ HDL-C） were also detected in patients with dyslipidemia. The correlation between the levels of lipid metabolism parameters and the levels of MCP-1,HO-1,APN and TNF-α was analyzed. Results The serum levels of MCP-1,HO-1,and APN in patients with dyslipidemia were significantly higher than those in healthy controls（ P 〈 0. 01）. Meanwhile the serum level of TNF-α in patients with dyslipidemia was significantly lower than that in healthy controls（ P〈 0. 01）. The serum level of HO-1 in patients with dyslipidemia was positively correlated with MCP-1 and LDL-C. The serum level of APN was negatively correlated with TNF-α,but positively correlated with LDL-C. The serum level of HO-1 had no correlation with MCP-1 in healthy control group. Conclusion MCP-1,HO-1,APN and TNF-α may participate in the occurrence and development of atherosclerosis and may be potential targets in the prevention and treatment of atherosclerosis.