HPLC法测定喉咽清口服液中齐墩果酸的含量

Determination of oleanolic acid in Houyanqing Oral Liquids by HPLC

目的建立HPLC-UV法测定酸水解后喉咽清口服液中的齐墩果酸的含量。方法以盐酸-乙醇溶液为水解溶剂,水浴加热回流1 h,再用石油醚（60～90℃）萃取得齐墩果酸;采用高效液相色谱法,以Ultimate XB-C18（4.6 mm×250 mm,5μm）色谱柱,乙腈-水（90∶10）作为流动相,流速为1.0 mL/min,柱温为30℃,检测波长为210 nm。结果齐墩果酸线性范围为2～12μg,平均回收率99.09%,RSD为1.83%。结论所建立方法操作简便、准确、可靠、重现性好,可用于喉咽清口服液中齐墩果酸的含量测定。

Objective To establish a method for the determination of oleanolic acid in Houyanqing Oral Liquids after being acid hydrolysis by HPLC. Methods The sample was hydrolyzed with hydrochloric acid- ethanol for 1 hours and extracted with petroleum ether（60-90℃）; the HPLC method was adopted. The Ultimate XB-C18column（4.6 mm×250 mm,5 μm） was used at the column temperature of 30℃. The mobile phase was composed of acetonitrile-water（90∶10） at a flow rate of 1.0 mL/min, the detected wavelength was set at 210 nm. Results The linear ranges of oleanolic acid was 2-12 μg, the average recovery rate was 99.09% with the RSD was 1.83%. Conclusion The developed method is so convenient and has better precision and repeatability. This method can be used to determine the content of oleanolic acid in Houyanqing Oral Liquids.