临床中央负压吸引设备插孔细菌检测研究

Study on detection of central negative pressure suction device terminal socket

目的:探讨中央负压吸引装置终端插孔消毒前后细菌检测情况,降低医院的交叉感染概率,为制定消毒管理规范提供依据。方法:对临床130个中央负压终端装置用安尔碘棉签进行螺旋式消毒,检验科人员分别对呼吸科48个、脑外科46个及小儿内科36个中央负压终端装置在消毒前后取样进行培养,将所取标本接种于无菌血琼脂平板培养基内,观察细菌生长情况,以检出细菌数量为污染标准。结果:临床设备中央负压吸引入终端插孔消毒后的污染率分别为呼吸科8.33%;脑外科6.52%;小儿内科5.56%,平均污染率为7.69%。检出的污染病原菌均为革兰氏阴性菌,消毒前后的污染率对比其差异有统计学意义(x2=14.08,P<0.05)。结论:采用安尔碘棉签消毒中央负压吸引终端口插孔,能够降低患者之间的交叉感染概率,可为患者提供安全的就医环境。

Objective：To discuss the bacteria detection before and after disinfection by using central negative pressure suction device terminal socket, reduce cross infection probability in hospitals and provide evidence for disinfection management specification. Methods：one hundred and thirty clinical central terminal devices with negative pressure were disinfected spirally with iodine swabs and among them, Department of respiration 48, Department of cerebral surgery 46, and Pediatrics 36, were sampled and sent to be cultured by clinical laboratory personnel before and after disinfection. The specimens were administered in a sterile blood AGAR plate culture medium, and the bacterial growth conditions were observed to detect the bacteria number as pollution standards. Results： The pollution rates of clinical equipment central pressure suction into the terminal socket after disinfection were： Department of respiration 8.33%; Department of cerebral surgery 6.52%; Pediatrics 5.56%, and the average pollution rate was 7.69%.The detected cases of pathogenic bacteria were gram-negative bacteria pollution, and contrasting the pollution rates before and after disinfection was statistically significant（x2=14.08, P＆lt;0.05）. Conclusion： Using based iodine swabs disinfection central pressure suction mouth terminal socket can reduce the cross infection between the patients with risk and provide patients with safe medical environment.