丁苯酞对大鼠急性脑缺血再灌注后Fas与FasL表达的影响

Effects of dl- 3- n- butylphthalide on expression of Fas and FasL of rats with focal cerebral ischemia- reperfusion

目的观察丁苯酞(NBP)对局灶性脑缺血再灌注损伤大鼠脑组织Fas和FasL表达的影响。方法 30只SD大鼠随机分为假手术组(Sham组)、脑缺血再灌注组(I/R组)和丁苯酞治疗组(NBP组)。线栓法制作大鼠大脑中动脉脑缺血再灌注模型,于缺血2 h再灌注24 h后断头取脑,免疫组织化学法检测缺血区脑组织Fas和FasL的蛋白表达,逆转录聚合酶链反应(RT-PCR)法检测缺血区脑组织Fas和FasL的mRNA表达。结果与Sham组相比,I/R组缺血区脑组织Fas和FasL的蛋白和mRNA的表达均显著增多;与I/R组相比,NBP组缺血区脑组织Fas和FasL的蛋白和mRNA的表达均显著下调,差异均有统计学意义(P<0.05)。结论丁苯酞对大鼠脑缺血再灌注损伤的保护作用,其机制可能与抑制Fas和FasL的表达有关。

Objective To investigate the effects of dl -3 -n -bu-tylphthalide on the expression of Fas and FasL in rats with focal cerebral ischemia -reperfusion.Methods Thirty rats were randomly assigned into three groups：Sham operation group （Sham group）,focal cerebral is-chemia-reperfusion group （ I/R group ） and dl-3-n-butylphthalide group （ NBP group ）.The middle cerebral artery occlusion reperfusion model was made by the suture method （ischemia for 2 hours, and reper-fusion for 24 hours ）.The level of Fas and FasL expression were meas-ured by immunohistochemistry and reverse transcription -polymerase chain reaction（RT-PCR）.Results Compared with sham group, the expressions of Fas and FasL as well as the mRNA were significantly up -regulated in I/R group （ P〈0.05 ）.Compared with I/R group, the ex-pressions of Fas and FasL as well as the mRNA were significantly down -regulated in the NBP group （ P〈0.05 ）.Conclusion The protection effect of dl-3-n-butylphthalide on rats with focal cerebral ischemia -reperfusion may be related to the inhibition of the expression of Fas and FasL.