摘要
目的考察七叶皂苷对大鼠肝微粒体的蛋白含量、CYP450酶总量和主要CYP450酶亚型(CYP1A2、CYP2D6、CYP3A4和CYP2C19)活性的影响。方法分别以0.9%NaCl和苯巴比妥作为空白组和阳性对照组,并以乙醇-5%葡萄糖溶液作为溶剂对照组,大鼠灌胃给予七叶皂苷(4.0 mg·kg-1·d-1),连续7 d,测定各组肝微粒体蛋白含量、CYP450蛋白含量以及CYP1A2、CYP2D6、CYP3A4及CYP2C19活性,并进行比较。结果与溶剂对照组相比,七叶皂苷给药组大鼠肝微粒体蛋白含量略增加,但差异无统计学意义(P>0.05);肝微粒体CYP450含量无明显差异。七叶皂苷给药后,CYP1A2和CYP3A4活性增加,但无统计学意义(P>0.05);CYP2D6和CYP2C19的活性没有改变。结论七叶皂苷对大鼠肝微粒体蛋白浓度、CYP450酶总量和CYP450酶亚型的活性均无影响。
Objective To study the effect of Aescine on the content of livermicrosome protein , cytochrome P450 （ CYP450 ） and the activity of CYP1A2, CYP2D6, CYP3A4 and CYP2C19 in cytomicrosome of rat liver.Methods Rats were given Aescine by oral administration （ 4.0 mg· kg-1 · d-1 ） for 7 days to detect the content of livermicrosome pro-tein and CYP450 and the activity of CYP1A2, CYP2D6, CYP3A4 and CYP2C19 in cytomicrosome taking 0.9%NaCl, ethanol-5%glucose so-lution and phenobarbital as blank , solvent and positive control group , re-spectively.Results Compared to solvent control group , Aescine in-creased the content of livermicrosome protein , however , it had no statisti-cal significance.Aescine had no effect on the content of CYP 450 , and the activity of CYP1A2, CYP2D6, CYP3A4 and CYP2C19 in solvent control group and Aescine group was comparable.Conclusions Aescine showed no effect on CYP 450 in rats.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2014年第7期620-622,631,共4页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(30772613)
