茶油中不饱和脂肪酸提纯工艺研究

Purification of unsaturated fatty acids from camellia oil

应用尿素包合法对茶油中的油酸、亚油酸进行纯化。通过阶梯式降温,使饱和脂肪酸乙酯、油酸乙酯、亚油酸乙酯依次分开。考察了第一阶段结晶温度、第二阶段结晶温度和尿素用量对油酸得率的影响。得到最佳工艺为:第一阶段结晶温度为5℃,第二阶段结晶温度为-10℃、尿素用量为1:1。此时,油酸和亚油酸的得率分别为86.19%和82.17%。结果表明,第一阶段结晶温度是影响纯化效果的主要因素。实现了对油酸、亚油酸的分离纯化,为茶油在食品及医药中的应用奠定了良好的基础。

The method of urea adduction fractionation was used to purify the oleic acid and linoleic acid in camellia oil. Saturated fatty acid ethyl esters, ethyl oleate and ethyl linoleate were separated by stepwise cooling. The effect of crystallization temperature in the first and the second stage and urea amount on the oleic acid yield was reviewed. The optimal technical parameter were the first stage crystallization tempera- ture was 5 ~C, and the second one was - 10 ~C, the urea amount was 1： 1. The yield of oleic acid was 86.19% and linoleic acid 82.17%. The result showed that the first stage crystallization temperature was the main factor to affect purification. The separation and purification of oleic acid and linoleic acid were realized, which settled a good foundation for the application of camellia oil in the food and pharmaceuticals.