玉米秸秆水解液发酵产微生物油脂的动力学模型研究

Kinetic model of microbial oils produced by fermenting corn stover hydrolysate

对玉米秸秆水解液发酵产微生物油脂动力学进行研究,基于Logistic方程和LuedekingPiret方程分别建立了微生物油脂发酵过程茵体生长和油脂合成随时间变化的数学模型,同时对试验值与模型进行了验证比较,应用气相色谱技术对微生物油脂中的不饱和脂肪酸成分进行分析。结果表明,以玉米秸秆为原料、深黄被孢霉为菌株,好氧间歇发酵,得到生物量为10.63g/L,油脂含量为49.53%。模型模拟计算结果与试验值能较好地吻合,该模型能较好地反映玉米秸秆水解液发酵产微生物油脂发酵的过程。

The dynamics of microbial oils fermented from corn stover hydrolysate was studied. The math- ematic model of thalli growth and microbial oils synthesis during the fermentation of microbial oils changed along with the time was built based on Logistic equation and Luedeking - Piret equation. The test value was validated with the model. The compositions of unsaturated fatty acid in microbial oils were analyzed by the gas chromatography. The results indicated that the corn stover was used as a raw materi- al, mortierella isabellina as a strain, after aerobic intermittent fermentation, the biomass and content of microbial oils reached 10.63 g/L and 49.53% respectively. A good coincidence between models calcula- tion values and experimental data was observed, therefore, the model equations can really reflect the process of the microbial oils fermentation.