期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

链霉菌HCCB10043中调控基因dptR1、dptR2及dptR3对A21978C合成的影响 被引量：2

Influence of regulatory genes dptR1, dptR2 and dptR3 on A21978C production in Streptomyces sp. HCCB10043
下载PDF
导出
摘要 目的研究A21978C生物合成基因簇内3个调控基因dptR1、dptR2及dptR3对A1978C合成的影响。方法利用同源重组方法敲除这3个调控基因,用HPLC检测突变株A21978C产量的变化。结果 dptR1和dptR2敲除后突变株A21978C产量与野生株HCCB10043相比变化不大,dptR3敲除后突变株不产A21978C。结论 dptR3对达托霉素的合成至关重要,是一个正调控基因。 Objective To investigate the influence ofA21978C biosynthesis gene clusters of 3 regulatory genes dptR1, dptR2 and dptR3 on synthesis ofA1978C. Methods dptR1, dptR2 and dptR3 were deleted by homologous recombination and the production ofA21978C in the corresponding mutants was detected via HPLC. Results There were three AdptR1 mutants, four AdptR2 mutants and two AdptR3 mutants obtained. The production of A21978C in AdptR1 and AdptR2 mutants was at a similar level to that of the wild-type strain, while the AdptR3 mutant lost its ability to produce A21978C. Conclusion dptR3 was essential forA21978C production and was a positive regulatory gene.
作者 靳旭 魏维 饶敏 陈玲玲 戈梅
机构地区 南京农业大学生命科学学院 上海来益生物药物研究开发中心 上海交通大学药学院
出处 《中国抗生素杂志》 CAS CSCD 北大核心 2014年第7期490-493,共4页 Chinese Journal of Antibiotics
基金 国家自然科学基金(81102354)
关键词 A21978C 调控基因 基因敲除 A21978C Regulatory gene Gene deletion
分类号 Q93 [生物学—微生物学]
  • 相关文献

参考文献8

  • 1Arbeit R D, Maki D, Tally F P, et al. The safety and efficacy skin and of daptomycin for the treatment of complicated skin-structure infections[J]. Clin Infect Dis, 2004, 38(12):1673-1681.
  • 2Fowler V G, Jr., Boucher Daptomycin versus standard HW, therapy Corey G R, et al for bacteremia and endocarditis caused by Staphylococcus aureus[J]. N Engl J Med, 2006,355(7):653-665.
  • 3Debono M, Barnhart M, Carrell C B, et al. A21978C, a complex of new acidic peptide antibiotics: isolationchemistry and mass spectral structure elucidation[J]. J Antibiot, 1987, 40(6): 761-777.
  • 4朱健,陈晓霞,许永锋,等.一种小链霉菌及其在达托霉素制备中的应用:中国,201010225330[P].2010-12-01.
  • 5饶敏,靳旭,阮丽军,罗敏玉,盛下放.小链霉菌HCCB10043产脂肽类抗生素的鉴定及其生物合成基因簇研究[J].中国医药工业杂志,2012,43(2):92-96. 被引量：7
  • 6Kieser T, Bibb M J, Buttner M J, et al. Practical Streptomyces genetics [M]. Norwich, England: The John Innes Foundation, 2000.
  • 7张艳娟,洪斌.链霉菌次级代谢调控机制进展[J].中国生物工程杂志,2004,24(12):39-42. 被引量：6
  • 8Shi T, Xie J. Regulation mechanisms underlying the biosynthesis of daptomycin and related lipopeptides[J]. J Cell Biochem, 2012, 113(3): 735-741.

二级参考文献30

  • 1Dalluge JJ, Smith S, Sanchez-Riera F. Potential of fermentation profiling via rapid measurement of amino acid metabolism by liquid chromatography-tandem mass spectrometry [J]. J ChromatogrA, 2004, 1043 (1) : 3-7.
  • 2Budai L, Ozohanics O, Ludanyi K, et al. Investigation of genetic variants of alpha-1 acid glycoprotein by ultra- performance liquid chromatography-mass spectrometry [J]. Anal Bioanal Chem, 2009, 393 (3) : 991-998.
  • 3Baranowska I, Magiera S. Analysis of isoflavones and flavonoids in human urine by UHPLC [J]. Anal Bioanal Chem, 2011,399 (9) : 3211-3219.
  • 4Rao M, Li Q, Feng L, et al. A new aminopeptidase inhibitor from Streptomyces strain HCCB10043 found by UPLC-MS [J]. Anal Bioanal Chem, 2011, 401 (2) : 699-706.
  • 5Debono M, Abbott B J, Molloy RM, et al. Enzymatic and chemical modifications of lipopeptide antibiotic A21978C: the synthesis and evaluation ofdaptomycin (LY146032) [J]. JAntibiot, 1988, 41 (8) : 1093-1105.
  • 6Debono M, Barnhart M, Carrell CB, et al. A21978C, a complex of new acidic peptide antibiotics: isolation,chemistry and mass spectral structure elucidation [J]. J Antibiot, 1987, 40 (6) : 761-777.
  • 7Miao V, Coeffet-Legal MF, Brian P, et al. Daptomycin biosynthesis in Streptomyces roseosporus: cloning and analysis of the gene cluster and revision of peptide stereochemistry [J]. Microbiology, 2005, 151 (5) : 1507-1523.
  • 8Paget MS, Chambedin L, Atrih A, et al. Evidence that the extracytoplasmic function sigma factor sigmaE is required for normal cell wall structure in Streptomyces coelicolor A3 (2) [J]. J Bacteriol, 1999, 181 (1) : 204-211.
  • 9Fowler VG, Boucher HW, Corey GR, et al. Daptomycin versus standard therapy for bacteremia and endocarditis caused by Staphylococcus aureus [J]. N Engl J Med, 2006, 355 (7) : 653-655.
  • 10朱健,陈晓霞,许永锋,等.一种小链霉菌及其在达托霉素制备中的应用:中国,201010225330[P].2010-12-01.

共引文献11

同被引文献11

  • 1BARANOWSKA I,MAGIERA S.Analysis of isoflavones and flavonoids in human urine by UHPLC[J].Anal Bioanal Chem,2011,399(9):3211-3219.
  • 2BUDAI L,OZOHANICS O,LUDANYI K,et al.Investigation of genetic variants of alpha-1 acid glycoprotein by ultra-performance liquid chromatography-mass spectrometry[J].Anal Bioanal Chem,2009,393(3):991-998.
  • 3RAO Min,LI Qiu-shuang,FENG Lei,et al.A new aminopeptidase inhibitorfrom Streptomyces strain HCCB10043 found by UPLC-MS[J].Anal Bioanal Chem,2011,401(2):699-706.
  • 4RAO Min,WEI Wei,GE Mei,et al.A new antibacterial lipopeptide found by UPLC-MS from an actinomycete Streptomyces sp.HCCB10043[J].Natural Product Research,2013,27(23):2190-2195.
  • 5ZHANG Xi,FERGUSON-MILLER S M,REID G E R.Characterization of ornithine and glutamine lipids extracted from cell membranes of Rhodobacter sphaeroides[J].J Am Soc Mass Spectrom,2009,20(2):198-212.
  • 6RAO Min,FENG Lei,RUAN Li-jun,et al.UPLC-MSBased metabolomic study of Streptomyces strain HCCB10043 under different p H conditions reveals important pathways affecting the biosynthesis of A21978C compounds[J].Analytical Letters,2013,46(15):2305-2318.
  • 7TAHARA Y,YAMADA Y,KONDO K.Antimicrobial activity of the ornithine-containing lipid isolated Gluconobacter cerinus[J].Agricultural and Biological Chemistry,1977,41(2):417-418.
  • 8GAO Jun-lian,WEISSENMAYER B,TAYLO A M,et al.Identification of a gene required for the formation of lyso-ornithine lipid,an intermediate in the biosynthesis of ornithine-containing lipids[J].Molecular Microbiology,2004,53(6):1757-1770.
  • 9LOPEZ-LARA I M,SOHLENKAMP C,GEIGER O.Membrane lipids in plant-associated bacteria:their biosyntheses and possible functions[J].Mol Plant Microbe Interact,2003,16(7):567-579.
  • 10GEIGER O,GONZALEZ-SILVA N,LOPEZ-LARA I M,etal.Amino acid-containing membranelipids in bacteria[J].Prog Lipid Res,2010,49(1):46-60.

引证文献2

二级引证文献1

中国抗生素杂志
2014年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部