杭白菊黄酮对高糖诱导的人脐静脉内皮细胞的影响

Influence of Flavon from Chrysanthemum Morifolium on Apoptosis of HUVECs

目的：探讨杭白菊黄酮对高糖诱导的人脐静脉内皮细胞（HUVEC）凋亡的影响，分析杭白菊黄酮对血管内皮细胞的保护作用。方法 HUVEC分正常对照组、高糖组（22mol/L）及低、中、高浓度杭白菊黄酮组（100、200、400mg/mL），作用24~72h后，显微镜观察细胞形态改变，MTT法检测细胞活力。结果不同浓度杭白菊黄酮组细胞存活率均显著高于高糖组（P〈0.05，P〈0.01），尤以400mg/mL高浓度杭白菊黄酮组作用最为显著（P〈0.01）。结论杭白菊黄酮对高糖诱导的HUVEC细胞凋亡有明显的抑制作用，且存在剂量依赖性，对血管内皮具有保护作用。

Objective To investigate the influence of flavon from Chrysanthemum morifolium on the apoptosis of human umbilical vein endothelial cells（HUVECs）. Methods HUVECs were treated with high glucose（22mol/L） and then with flavone from Chrysanthemum morifolium of different concentrations（100,200,and 400mg/mL） for 72h. The cell morphological changes were observed by microscope and cell proliferation was determined by MTT chromatometry. Results The cell viability in flavon groups with different concentrations were higher than that in high glucose group（P〈0.05 or P〈0.01）,with the most increase in 400mg/mL flavon group（P〈0.01）. Conclusion Flavonoids from Chrysanthemum morifolium can inhibit the high-glucose-induced apoptosis of HUVECs in a dose-dependent manner. This indicated that flavon from Chrysanthemum morifolium have protective effects on HUVECs.