兔机械性肠梗阻模型的建立

Establishment of Rabbit Model of Mechanical Intestinal Obstruction

目的建立可用于研究机械性肠梗阻的家兔动物模型。方法选取54只家兔,随机分为正常对照组、低位小肠梗阻组(实验1组)、结肠梗阻组(实验组),每组18只,以头皮针及丝线结扎回肠末端及乙状结肠方法建立完全性单纯机械性肠梗阻模型,术后24、48、72h,测量腹围、梗阻肠管周径、肠道内压及腹腔内压。结果实验组与对照组比较,术后24、48h腹围无明显增大(P>0.05),术后72h腹围增大(P<0.05);实验组梗阻肠道周径、肠道内压、腹腔内压术后24h无明显增加(P>0.05),术后48h增加明显(P<0.05),术后72h增加显著(P<0.05)。结论该动物模型符合机械性肠梗阻的临床表现及肠道变化,可以用于研究机械性肠梗阻。

Objective To establish a rabbit model of mechanical intestinal obstruction （MIO）. Methods Fifty-four rabbits were randomly divided into control group（n=18）,small bowel obstruction group（n=18）,and large bowel obstruction group（n=18）. Ligation of the ileum（terminal part） and sigmoid was achieved by using scalp needle and 7-0 thread to establish simple MIO model,respectively. At 24h,48h,72h after modeling, abdominal circumference, perimeter of obstructed intestine,intestinal pressure and intra-abdominal pressure were measured. Results Compared with control group, the abdominal circumference in small bowel obstruction group and large bowel obstruction group was not significantly different（P〉0.05） at 24 and 48 h after modeling,but increased at 72h（P〈0.05）;the perimeter of obstructed intestine,intestinal pressure and intra-abdominal pressure in small bowel obstruction group and large bowel obstruction group did not increase at 24h after modeling,but increased at 48 and 72h（P〈0.05）. Conclusion The animal model is in accordance with clinical manifestations and intestinal changes of MIO. It can be used for MIO studies.