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拟南芥原生质体瞬时表达快速验证重组酶外源基因删除

Recombinase-mediated Marker Gene Excision in Transient Gene Expression System of Arabidopsis Mesophyll Protoplasts
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摘要 通过在拟南芥原生质体中瞬时表达重组酶删除系统,以拟南芥热激蛋白Hsp18.2基因的启动子诱导加入马铃薯StLs1基因第2个内含子的重组酶表达,证明瞬时转化质粒DNA中位于识别位点之间的基因元件可以被有效删除。建立了一种快速验证重组酶外源基因删除系统的方法。 In the present study, we used transient gene expression system of Arabidopsis mesophyll protoplasts to test and compare site-specific recombination constructs, which the expression of recombinase was induced by the promoter for heat-shock proteins Hsp18.2, developed a simple and convenient system for rapid assessment of site-specific recombination constructs.
作者 池俊杰 戴绍军 魏建华 王宏芝
机构地区 东北林业大学盐碱地生物资源环境研究中心 北京农业生物技术研究中心
出处 《生物技术通报》 CAS CSCD 北大核心 2014年第7期86-92,共7页 Biotechnology Bulletin
基金 国家高技术研究发展计划(2013AA102702)
关键词 位点特异性重组系统 热激蛋白Hsp18 2启动子 拟南芥原生质体瞬时表达 Site-specific recombination system Promoter for heat-shock proteins Hspl$.2 Transient gene expression system of Arabidopsis protoplast
分类号 Q943.2 [生物学—植物学]
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参考文献26

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二级参考文献21

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生物技术通报
2014年 第7期

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