OsPT8过量表达提高转基因烟草的耐低磷能力

Overexpression of OsPT8 Gene to Enhance the Pi Deficiency Tolerance of Transgenic Tobacco

高亲和磷转运蛋白负责植物在低磷条件下吸收和转运磷酸盐,对植物的生长发育至关重要。将水稻中关键的高亲和磷转运蛋白基因OsPT8(A high affinity phosphate transporter gene OsPht1;8,以下简称OsPT8)通过农杆菌介导的方法转入烟草云烟87,以转基因烟草和野生型(云烟87)为材料,设置正常供磷(1 mmol/L Pi)和低磷(0.1 mmol/L Pi)两个处理的沙培试验,检测烟株地上部和地下部的生物量、全磷及有效磷的含量,分析烟草高亲和磷转运蛋白家族基因(NtPT1和NtPT2)的表达差异。结果显示,低磷条件下,OsPT8过量表达转基因株系生物量均显著高于野生型;在正常供磷和低磷条件下,OsPT8过量表达烟草株系全磷含量和有效磷含量均显著高于野生型,这表明高亲和磷转运蛋白基因OsPT8可以提高转基因烟草的耐低磷能力。RT-PCR和Q-PCR结果显示,转基因株系显著提高了烟草高亲和磷转运蛋白基因NtPT1和NtPT2的表达量,表明OsPT8对烟草磷吸收和转运的影响是通过OsPT8基因和烟草NtPT1、NtPT2基因等一个复杂的过程起作用的。

Plant phosphate transporters（PTs）are active in the uptake of inorganic phosphate（Pi）from the soil and it＆#39;s translocation within the plant. In this work, the key phosphate transporter gene OsPT8 in rice was transformed into tobacco（Yunyan 87）by Agrobacterium-mediated method. We set two treatments-normal P（1 mmol/L Pi）and low P（0.1 mmol/L Pi）by transgenic and wild-type tobacco as materials, detecting biomass and contents of P in shoots and roots, and analyzing the expression of phosphate transporter family gene（NtPT1 and NtPT2）. The results show that the biomass, the contents of total P and Pi of shoots and roots in transgenic line increased significantly compared with wild type under low Pi treatment, this data suggest that overexpression of OsPT8 can enhance the Pi deficiency tolerance of transgenic tobacco;the data of gene expression of NtPT1 and NtPT2 suggest that overexpression of OsPT8 enhance the Pi deficiency tolerance of transgenic tobacco caused by OsPT8 and other phosphate transporters.