氟西汀对卒中后抑郁大鼠行为学及海马神经元凋亡的影响

Effects of Fluoxertin on the Ethology and Apoptosis of Hippocampal Neurons in Rats with Post-stroke Depression

目的探讨以氟西汀为代表的SSRIs对卒中后抑郁(post-stroke depression,PSD)大鼠行为学和神经元凋亡的影响。方法用大脑中动脉线栓法(MCAO)建立大鼠局灶性脑缺血模型,再结合慢性不可预见的温和性应激(chronic unpredictable mild stress,CUMS)和孤养法建立PSD模型,加以10 mg·kg-1氟西汀干预。比较各组大鼠体质量与糖水消耗量;旷野实验测定直立活动和水平活动得分;流式细胞仪分析神经元细胞的凋亡率。结果应激14 d后,与PSD组大鼠比较,氟西汀干预组大鼠体质量和糖水消耗比例低(P<0.05或P<0.01),水平和垂直试验得分下降(P<0.01),海马神经元凋亡率较高(P<0.01)。结论氟西汀对PSD有一定的治疗作用。

OBJECTIVE To discuss the effects of fluoxertin as the representative of SSRIs on the ethology and apoptosis of hippocampal neurons in rats with post-stroke depression（PSD）. METHODS The focal cerebral ischemia novel model was set up by blocking the middle cerebral artery（MCAO） and then the rats in model group were separately raised and put into chronic unpredictable mild stress（CUMS） forinducing the PSD model. Rats in PSD＋fluoxertin group were administrated 10 mg·kg-1 fluoxertin, ip. Weights, sugar-water consumption, locomotor activity and apoptosis rate of hippocampal neurons were detected respectively. RESULTS On the 14th day after CUMS, the PSD group showed significantly less weights, locomotor activity and sugar-water consumption（P〈0.05 or P〈0.01） and higher apoptosis rate of hippocampal neurons compared with the PSD＋fluoxertin group（P〈0.01）. CONCLUSION Fluoxetine is effective for the treatment of PSD.