通光藤化学成分及抗肿瘤活性研究

Chemical Constituents and Anti-tumor Activity of Marsdenia Tenacissima

目的提取分离并筛选通光藤中具有抗肿瘤活性的化学成分,并研究其化学结构。方法系统溶剂法对通光藤药材进行化学部位分离,并利用色谱法对正丁醇部位中的化学成分进行分离和纯化,利用波谱学方法鉴定其化学结构。MTT法筛选所得化学成分体外对8种人癌细胞增殖的抑制作用。结果在通光藤正丁醇部位中得到3个化学成分单体分别为:牛奶菜醇(化合物1)、通光藤苷元B(化合物2)、大叶牛奶菜苷丁(化合物3),化合物2对于MGC-803肿瘤细胞抑制作用较强,IC50为80.3μg·mL-1,而化合物3对于ISMMC-7721肿瘤细胞液表现出较强抑制作用,IC50为40.7μg·mL-1。结论化合物2为首次在该植物中分离到的苷元成分。化合物2、3体外对肿瘤细胞具有显著的抑制活性。

OBJECTIVE To isolate and screen the chemical constituents which have anti-tumor activity from Marsdenia tenacissima, and to study their structure. METHODS Separate chemical site by systemic solvent extraction, then isolate and purify the parts of n-butanol by chromatography to identify their chemical structure by spectroscopic methods. Finally, from the obtained constituents, screened the bioactive compounds which had inhibitory effects on eight kinds of human cancer cells proliferation by MTT assay. RESULTS Three compounds were separated from n-butanol extract portion of Marsdenia tenacissima, and were identified as conduritol（compound 1）, tenacigenin B（compound 2）, marsdekoiside（compound 3）. Compound 2 had a strong inhibitory effect to MCG-803 tumor cell, ICs0 was 80.3μg·mL-1. Compound 3 had a strong inhibitory effect to ISMMC-7721 tumor cell, ICs0 was 40.7μg·mL-1. CONCLUSION Compound 2 was first aglycone isolated from Marsdenia tenacissima. Compounds 2 and 3 have significant inhibitory activity to tumor cells in vitro.