HPLC测定保健食品中微胶囊型维生素A

Determination of Vitamin A in the Form of Microcapsule in Functional Food by HPLC

目的 建立测定保健食品中微胶囊型维生素A含量的反相高效液相色谱法。方法 以适宜浓度氨水溶液破坏保健食品中微胶囊型维生素A的微胶囊结构,再以有机溶剂提取。采用Agilent ZORBAX SB-C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇为流动相,流速1.0 mL.min 1,检测波长为325 nm,柱温35℃。结果 维生素A在1.41--56.4μg.mL 1内线性关系良好（r=1.000）;平均回收率为97.5%（RSD=1.9%）。结论 本法方法简单、准确,重现性好,适用于保健食品中微胶囊型维生素A的测定。

OBJECTIVE To develop an HPLC method for determination of vitamin A in the form of microcapsule in functional food. METHODS Microcapsule structure was disrupted by suitable ammonia solution and vitamin A was extracted by organic solutions. The chromatographic separation was achieved on an Agilent ZORBAX SB-C18 column（250 mm×4. 6 mm, 5 μm） with methanol as the mobile phase. The detection wavelength was at 325 nm, flow rate 1.0 mL-min-1 and the column temperature 35℃. RESULTS The linear range of vitamin A was 1.41-56.4μg-mL-1（r=1.000）. The average recovery of vitamin A was 97.5%（RSD=1.9%）. CONCLUSION The established method was simple, accurate and with good reproducibility, suitable for content determination of vitamin A in functional food.