小菜蛾不同品系羧酸酯酶与乙酰胆碱酯酶的活性测定

Activity Determination of CarE and AChE to Different Strains of Plutella xylostella

为了明确小菜蛾抗阿维菌素品系、抗高效氯氰菊酯品系和抗复配剂(阿维菌素和高效氯氰菊酯复配)品系与羧酸酯酶(CarE)活性和乙酰胆碱酯酶(AChE)活性的相关性,为小菜蛾抗性治理提供依据,采用改进的Ellman胆碱酯酶活性测定方法等对小菜蛾3个品系的CarE活性和AchE活性进行测定。结果表明:抗阿维菌素品系、抗高效氯氰菊酯品系和抗复配剂品系的CarE比活力和AChE比活力分别是敏感品系的1.005 6倍和1.094 4倍、0.829 0倍和1.012 0倍、0.836 3倍和1.054 2倍;抗复配剂品系的CarE活性和AChE活性均介于抗阿维菌素品系和抗高效氯氰菊酯品系之间。

In order to explore the relationship of CarE and AChE to resistant strains avermectin,beta-cypermethrin and mixtures （abamectin and beta-cypermethrin complex）of P .xylostella,and provide a reference for the resistance management,the enzyme activities of CarE and AChE were determined by improved Ellman cholinesterase activity determination method. The results showed that the specific activities of CarE and AChE of resistant strains avermectin, beta-cypermethrin and mixtures were respectively 1.005 6 times,1.094 4 times,0.829 0 times,1.012 0 times,0.836 3 times and 1.054 2 times of sensitive strains;The activities of CarE and AChE of the mixtures were between that of the avermectin strain and beta-cypermethrin strain.