HPLC法测定蒙药消肿九味散中大黄酸、大黄素、大黄酚含量

Determination of Rhein,Rmodin and Chrysophanol in Mongolian Xiaozhong Jiuwei Powder by HPLC

目的：测定蒙药消肿九味散中大黄酸、大黄素、大黄酚3种活性成分的含量。方法：采用高效液相色谱（HPLC）法,Dimaonsil-C18（250 mm×4.6 mm,5μm）色谱柱;以甲醇-0.1%磷酸溶液（85∶15）为流动相;流速：1.0 mL·min-1;检测波长：254 nm,柱温为25℃。结果：大黄酸、大黄素、大黄酚3种成分分别在0.101 8~0.508 8μg、0.117 0~0.585 0μg、0.256 0~1.280 0μg范围内与其峰面积呈良好的线性关系,相关系数均大于0.9997;且样品中3种成分的平均加样回收率（n=6）分别为98.98%、99.54%、97.36%,RSD均小于1.32%。结论：建立的含量测定方法简便、快速、灵敏、准确,可以从该制剂中原料药材君药的角度用于该制剂的质量控制。

Objective ： To determinate the contents of three active ingredients in Mongolian medicine of Xiaozhong Jiuwei Powder, including rheinic acid, rheum emodin and chrysophanol. Methods：High Pressure Liquid Chromatography（HPLC） was adopted, using the Dimaonsil - C18 （250mm × 4.6mm, 5μm） as chromatographic column with 25 ℃ , methanol - 0. 1% phosphoric acid solution（85：15）as mobile phase. The flow rate was 1.0 mLrain-1 and detection wavelength was 254 nm. Results：The three ingredients of rheinic acid, rheum emodin and chrysophanol showed good linear relation with their peak areas within the limits of 0. 1018-0.5088μg（ r = 0. 9997 ） ,0.1170 - 0. 5850 μg（ r = 0. 9997 ） and 0. 2560 - 1. 2800 μg（ r = 0. 9998 ） , respectively. All their correlation coefficients were greater than 0.9997. The mean recovery of 98.45% （ 1.32% ） for rheinic acid,99.60% （ 1.09 % ）for rheum emodin and 97.36 % （ 1.17 % ）for c hrysophanol were obtained （ n = 6）, and all the RSD values were less than 1.32%. Conclusion：The method established in this study is simple,high accuracy,precision and specific,and thus it can be used to control the quality of preparation perspective from monarch drug in a crude medicinal materials of a prescription.