十一味益肾降糖片对Ⅲ期糖尿病肾病大鼠肾损伤干预的实验研究

Experiment Study on Shiyiwei Yishen Jiangtang Tablet on Kidney Damage of Ⅲ Stage DN Rats

目的:探讨十一味益肾降糖片对Ⅲ期糖尿病肾病(DN)大鼠肾损伤的干预作用。方法:采用高糖高脂饮食加链脲佐菌素(STZ)腹腔注射的方法建立Ⅲ期DN大鼠模型,设立空白组(A组)、模型组(B组)、十一味益肾降糖片组(C组)、厄贝沙坦治疗组(D组)。灌胃12周后,检测各组大鼠空腹血糖(FBG)、肾肥大指数(KW/BW)、24 h尿蛋白定量(24-hrUP)、血清胱抑素(CysC)、肾小球硬化指数等指标,取右肾制作病理切片,观察肾脏病理改变。结果:十一味益肾降糖片可改善DN大鼠的一般状况,降低FBG、KW/BW、24-hrUP、CysC等指标;抑制肾小球毛细血管基底膜增厚、系膜基质增生及细胞外基质合成。结论:十一味益肾降糖片可降低Ⅲ期DN大鼠FBG、KW/BW、24-hrUP、CysC等指标;减轻肾脏病理损害,保护肾脏功能。

Objective：To discuss the intervention effect of Shiyiwei Yisben Jiangtang Tablet on kidney damage of m stage DN rats. Methods：The DN rat model was established by high suger and high fat food with STZ injection in abdomen. There were blank group（ A group）, model group （ B group ）, Shiyiwei Yishen Jiangtang Tablet group （ C group ）, Irbesartan group （ D group ）. 12 weeks after gavage ,FBG, KW/BW ,24 -hrUP, CysC and other factors were detected and the pathological changes were observed. Results ： Shiyiwei Yishen Jiangtang Tablet can relieve the common conditions of DN rats and reduce FBG, KW/BW, 24 - hrUP, CysC and other factors. It also can inhibit glomerular basement membrane thickening mesangial matrix proliferation and extracellular matrix synthesis. Conclusion：Shiyiwei Yishen Jiangtang Tablet can reduce FBG, KW/BW,24 -hrUP, CysC and other factors of DN rats in Ⅲ stage,relieve kidney damage and protect kidney functions.