双氯芬酸二乙胺乳胶剂的含量测定

Content Determination of Diclofenac Diethylamine Emulgel

目的建立测定双氯芬酸二乙胺乳胶剂含量的高效液相色谱法。方法以甲醇-4％冰醋酸（70：30）为流动相，色谱柱为CNW C18柱（150mm×4．6mm，5μm），检测波长为276nm。结果双氯芬酸钠质量浓度在51．600～1290．0μg／mL范围内与峰面积呈良好线性关系，双氯芬酸钠的平均回收率为98．53％，RSD为0．71％。结论该方法简单、灵敏度高，可用于双氯芬酸二乙胺乳胶剂的含量测定。

Objective To establish a HPLC method for the content determination of Diclofenac Diethylamine Emulgel. Methods The separation was performed on the CNW C18 column（150 mm×4.6mm,5μm）.The mobile phase was methanol-4% glacial acetic acid solution（70：30）. The UV detection wavelength was set at 276 nm. Results The mass concentration of diclofenac diethylamine in the range of 51. 600- 1 290.0 μg/mL had a good linear relation with the peak area,the average recovery rate of diclofenac diethylamine was 98.53%, RSD =0. 71%. Conclusion This method is simple, highly sensitive and can be used for the content determination of Diclofenac Diethylamine Emulgel.