子痫前期患者胎盘中肝X受体α与胆固醇调节元件结合蛋白-1c表达及其意义

Expression and significance of LXRα and SREBP-1c in placenta of pre-eclampsia

目的研究子痫前期患者胎盘中肝X受体α(LXRα)及其靶基因胆固醇调节元件结合蛋白-1c(SREBP-1c)的表达及相关性,探讨两者在子痫前期中的意义。方法 2011年10月至2012年8月在福建医科大学附属第一医院观察子痫前期组30例(轻度14例和重度16例)和正常妊娠组30例。采用逆转录-聚合酶链反应(RT-PCR)及免疫组化技术(IHC)检测胎盘组织中LXRα及SREBP-1c的mRNA和蛋白表达水平。结果 RT-PCR和IHC结果显示正常妊娠组和轻、重度子痫前期组胎盘中LXRα、SREBP-1c的mRNA和蛋白的表达均逐步升高,且差异有统计学意义(P<0.01或P<0.05)。LXRαmRNA与LXRα蛋白表达呈正相关(r=0.417,P<0.01)。SREBP-1c mRNA与SREBP-1c蛋白表达呈正相关(r=0.611,P<0.01)。LXRα蛋白与SREBP-1c蛋白表达呈正相关(r=0.602,P<0.01)。结论子痫前期患者胎盘组织中LXRα和SREBP-1c的转录和翻译水平显著高于正常妊娠,重度子痫前期表达水平更高。

Objective To evaluate the expression and correlations of liver X receptor alpha（LXRα） and its target gene sterol regulatory element-binding protein-1c （SREBP-1c） in placenta of pre-eclampsia（PE） and their significance in PE.Methods Between October 2011 and August 2012 in the First Affiliated Hospital of Fujian Medical University observed PE group 30 cases（mild 14cases and severe 16cases） and normal pregnancy group 30 cases. The level of mRNA and protein of LXRα and SREBP-1c were analyzed by reverse transcription-polymerase chain reaction（RT- PCR）and immunohistochemistry （IHC） in the placenta.Results RT-PCR and IHC all show that the expressions of mRNA and protein of LXRα and SREBP-1c increased gradually with significantly different levels among normal pregnancy，mild pre-eclampsia and severe pre-eclampsia groups（P〈0.01 or P〈0.05）.There were positive correlations between the expression of LXRα mRNA and LXRα protein（r=0.417,P〈0.01）.There were positive correlations between the expression of SREBP-1c mRNA and SREBP-1c protein（r=0.611,P〈0.01）.There were positive correlations between the expression of LXRα protein and SREBP-1c protein（r=0.602,P〈0.01）.Conclusion The transcription and translation of LXRα and SREBP-1c in placenta of PE are significantly higher than that of normal pregnancy,which are highest in severe PE.