妊娠期糖尿病患者外周血高迁移率族蛋白1表达水平检测及其临床意义

The detection of HMGB1 in peripheral blood in gestational diabetes mellitus patients

目的 检测妊娠期糖尿病（GDM）患者外周血高迁移率族蛋白1（HMGB1）的表达水平，探讨HMGB1在GDM患者胰岛素抵抗中的作用。方法 选择2011年1月至12月在南京医科大学附属苏州医院建卡，孕24～28周产前检查被诊断患有GDM的孕妇100例为研究对象，另选择同期相应年龄的正常妊娠妇女50例作为对照组。取两组孕妇外周血，测定空腹血糖、胰岛素、HMGB1、IL-6水平。结果 GDM孕妇组胰岛素抵抗指数（HOMA-IR）为5.89±2.04，比正常孕妇组2.08±1.08高（P〈0.01）；GDM孕妇组HMGB1为（8.57±1.26） μg/L、IL-6为（10.67±2.33）ng/L，表达水平均高于正常孕妇组HMGB1（6.11±0.78）μg/L、 IL-6（6.33±1.00 ）ng/L（P〈0.01）。GDM组HOMA-IR与HMGB1、IL-6的质量浓度水平之间r分别为0.7713、0.7055、0.7147。结论 检测GDM患者外周血HMGB1水平，有助于探讨HMGB1在GDM发病过程中的病理生理作用，也为寻找GDM的治疗靶点提供新的思路。

Objective To detect the expression levels of high mobility group box chromosomal protein 1 （HMGB1） in peripheral blood in gestational diabetes mellitus （GDM） patients. To identify the relationship between HMGB1 and insulin resistance index （ HOMA-IR ） in GDM patient. Methods Select pregnant women who prenatal care in Suzhou Hospital Affiliated to Nanjing Medical University between January 2011 and December 2011. The recruitment of pregnant diagnosed with GDM between 24～28 weeks gestational age, A total of 100 cases. Recruitment of another 50 cases of normal pregnant women as the study control. The levels of fasting blood glucose, insulin, HMGB1, IL-6 levels were detected in peripheral blood. Results The insulin resistance index （ HOMA-IR ） in GDM pregnants 5.89±2.04 were higher than that in normal pregnant women2.08±1.08 （P〈0.01 ）; The concentration of HMGB1 and IL-6 in GDM patients were higher than that in health control group （P〈0.01）; and positive correlations r are 0.7713，0.7055 and 0.7147 respectively, between insulin resistance index （ HOMA-IR ） and the expression levels of HMGB1, IL-6 in GDM group.