氟苯尼考在美国红鱼体内的药代动力学和组织分布

Tissues Distribution and Pharmacokinetics of Florfenicol in Sciaenops ocellatus

在水温20±2℃的条件下,按10 mg/kg的剂量通过腹腔注射和口灌方式,以液-质联用法研究氟苯尼考在美国红鱼(Sciaenops ocellatus)体内的药代动力学和组织分布,所得药时数据用DAS3.0软件分析。结果显示,美国红鱼腹注氟苯尼考后血药经时符合一级吸收二室模型,而口灌氟苯尼考则符合一级吸收一室模型;腹腔注射组,美国红鱼血浆、肝脏、肾脏、肌肉的氟苯尼考出峰时间(tp)分别为0.5、4、0.5、4 h,峰浓度(ρmax)分别为15.47、9.90、6.83、2.73μg/g,药时曲线下面积(AUC)分别为394.10、105.66、157.37、120.01 mg/(L·h),消除半衰期分别为45.39、7.42、148.25、39.22 h;口灌组,美国红鱼血浆、肝脏、肾脏、肌肉的氟苯尼考出峰时间(tp)分别为2、1.5、4、4 h,峰浓度(ρmax)分别为6.31、5.44、9.10、5.12μg/g,药时曲线下面积(AUC)分别为136.21、162.32、213.32、157.37 mg/(L·h),消除半衰期t1/2β分别为12.08、27.9、14.85、38.53 h。结果表明,氟苯尼考注射给药在美国红鱼体内的吸收快于口灌给药,腹注给药氟苯尼考除在肝脏中的消除快于口灌给药外,在血浆、肌肉和肾脏中的消除均慢于口灌给药。

The distribution and pharmacokinetics of florfeniol were investigated in tissues of Sciaenops ocellatus with enterocoelia injection and oral administration ration of 10 mg/kg body weight at water temperature of （20 ± 2） ℃ by HPLC-MS method. Pharmacokinetics parameters were calculated by DAS3.0 software. The florfenicol concentration-time data in plasma following the injective administration was best described by two-compartment model with first-order absorption, while one-compartment open model was the best described by the oral administration group. The major pharmacokinetic parameters were as follows： The time of peak （tp） of florfenicol in plasma, liver, kidney, muscle of Sciaenops ocellatus with enterocoelia injection were 0.5, 4, 0.5, 4 h, and the maximum concentration （ρmax） of that were 15.47, 9.90, 6.83, 2.73 μg/g, and the area under the concentration-time curve （AUC） were 394.10, 105.66, 157.37, 120.01 mg/（L.h）, and the elimination half-life （tl/2β） were 45.39, 7.42, 148.25, 39.22 h, respectively. The time of peak （tp） of florfenicol in plasma, liver, kidney,and muscle of Sciaenops ocellatus with oral administration were 2, 1.5, 4, 4 h, and the maximum concentration （ρmax） of that were 6.31, 5.44, 9.10, 5.12 μg/g, and the area under the concentration-time curve （AUC） were 136.21, 162.32, 213.32, 157.37 mg/（L·h）, and the elimination half-life （t1/2β） were 12.08, 27.9, 14.85, 38.53 h, respectively. It is suggested that FF was absorbed quicker, and eliminated faster in all tested tissues but eliminated slower in plasma, muscle and kidney after enterocoelia injection than that after oral administration.