骨髓单个核细胞移植在脑梗死小鼠脑内分化为星形胶质样细胞的研究

Differentiation of transplanted bone marrow mononuclear cells into astrocytes in brain of ischemic stroke mice

目的探讨骨髓单个核细胞(BMMNC)移植对大脑中动脉栓塞小鼠的治疗作用以及脑内分化情况。方法选择41只雄性昆明小鼠,采用线栓法制备大脑中动脉栓塞模型后,将制模成功的34只小鼠随机分为溶剂组和BMMNC移植组(移植组),每组17只。分别于模型成功后1、3、7、14和28d采用改良神经功能缺损评分(mNSS)评价小鼠神经损害严重程度,免疫荧光双标法观测BMMNC脑内分化情况。结果与溶剂组比较,移植组1、3、7、14和28d各时间点mNSS明显降低[(12.54±1.50)分vs(13.02±1.52)分、(9.52±1.25)分vs(11.17±1.05)分、(7.92±1.24)分vs(10.75±1.06)分、(5.82±1.23)分vs(7.93±1.23)分、(4.92±1.25)分vs(6.83±1.12)分,P<0.05];移植组梗死皮质及海马齿状回区5-溴脱氧尿嘧啶核苷和胶质纤维酸性蛋白双标阳性细胞。结论BMMNC移植在脑梗死小鼠脑内分化为星形胶质样细胞,能显著改善脑梗死小鼠神经功能。

Objective To study the therapeutic effect of transplanted bone marrow mononuclear cells （BMMNC） on middle cerebral artery （MCA） embolism and their differentiation in brain of ischemic stroke mice.Methods Forty-one male Kunming mice were included in this study.A MCA embolism model was established by Longa occlusion.Thirty-four mice in the established model were randomly divided into vehicle group and BMMNC transplantation group （17 in each group）.Nerve injury was assessed according to their mNSS score on days 1,3,7,14,28,respectively.Differentiation of BMMNC in brain of mice was observed with double immunofluorescence staining.Results The mNSS score was significantly lower in BMMNC group than in vehicle group at different time points （12.54±1.50 vs 13.02±1.52,9.52±1.25 vs 11.17±1.05,7.92± 1.24 vs 10.75±1.06,5.82±1.23 vs 7.93±1.23,4.92±1.25 vs 6.83±1.12,P＜0.05）.Brdu and GFAP double positive cells were detected in infracted cortex and hippocamal dentate gyrus of BMMNC group.Conclution Transplanted BMMNC can differentiate into astrocytes and effectively improve the neurological function in ischemic stroke mice.