期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

吞噬凋亡淋巴细胞的树突状细胞诱导高分泌白细胞介素-10的调节性B细胞的生成 被引量：1

Dendritic cells phagocytized apoptotic lymphocytes induce the development of regulatory B cells with high secretion of interleukin-10
下载PDF
导出
摘要 目的探讨未成熟树突状细胞(imDC)吞噬经补骨脂素长波紫外线(PUVA)处理的小鼠脾B淋巴细胞(PUVA-SP)后,将B淋巴细胞诱导为高分泌白细胞介素(IL)-10(IL-10+)的调节性B细胞的可行性。方法培养小鼠骨髓来源DC,小鼠脾淋巴细胞经PUVA光源照射得到PUVA-SP,在体外将PUVA-SP与骨髓来源的imDC共同培养,后者吞噬PUVA-SP后形成PUVA-SP DC。应用CD19磁珠分选小鼠脾脏B淋巴细胞,将不同的DC与B淋巴细胞共同培养48 h。采用酶链免疫吸附试验(ELISA)检测B淋巴细胞、imDC、imDC+B淋巴细胞、PUVA-SP DC、PUVA-SP DC+B淋巴细胞的培养上清液中干扰素(IFN)-γ、转化生长因子(TGF)-β、IL-12p70、IL-10等细胞因子的含量。流式细胞仪分析B淋巴细胞、imDC+B淋巴细胞、mDC+B淋巴细胞、PUVA-SP DC+B淋巴细胞共培养后IL-10+调节性B细胞数量。结果与其他4组细胞培养液比较,PUVA-SP DC+B淋巴细胞上清液中的IL-10含量明显升高(均为P<0.05)。与其他各组相比,PUVA-SP DC+B淋巴细胞中IL-10+调节性B细胞数量明显增多。结论 PUVA-SP DC可以诱导脾脏B淋巴细胞分化为IL-10+调节性B细胞。 Objective To investigate the feasibility of immature dendritic cells （imDC）phagocytized psoralen ultraviolet A （PUVA）-treated splenic lymphocytes （PUVA-SP DC）in mice inducing B lymphocytes to be regulatory B cells （Breg）with high secretion of interleukin （IL）-10 （IL-10 ＋Breg）. Methods Bone marrow-derived DC of mice was cultured. Spleen lymphocytes of mice were isolated and treated by PUVA,and turned to be PUVA-SP. The bone marrow-derived imDC was co-cultured with PUVA-SP in vitro to obtain PUVA-SP DC. Splenic B lymphocytes of mice were separated by anti-CD19 magnetic beads and co-cultured with different kinds of DC for 48 hours. The levels of interferon （IFN ）-γ,transforming growth factor （TGF）-β,IL-12p70,and IL-10 in the culture supernatant of B lymphocytes,imDC,imDC＋B lymphocytes, PUVA-SP DC and PUVA-SP DC ＋B lymphocytes were measured by enzyme-linked immune absorbent assay （ELISA）. The accounts of IL-10 ＋Breg in B lymphocytes,imDC＋B lymphocytes,mDC＋B lymphocytes and PUVA-SP DC＋B lymphocytes were detected by flow cytometry. Results Compared with the other 4 groups, the level of IL-10 in cell culture supernatant of PUVA-SP DC＋B lymphocytes was significantly higher （all in P〈0.05）. Compared with the other groups,the account of IL-10 ＋Breg in PUVA-SP DC＋B lymphocytes was＆amp;nbsp;significantly higher. Conclusions PUVA-SP DC can induce splenic B lymphocytes to differentiate into IL-10 ＋Breg.
作者 魏玉香 王晓刚 肖漓 周文强 郑德华 于涛 石炳毅
机构地区 解放军第
出处 《器官移植》 CAS CSCD 2014年第4期237-241,共5页 Organ Transplantation
基金 国家自然科学基金(81370578)
关键词 树突状细胞 体外光化学方法 调节性B细胞 白细胞介素-10 Dendritic cell Photochemical method in vitro Regulatory B cell Interleukin-10
分类号 R617 [医药卫生—外科学]
  • 相关文献

参考文献9

  • 1Benichou G, Tocco G. The road to transplant tolerance is paved with good dendritic cells [ J ]. Eur J Immunol, 2013, 43 (3): 584-588.
  • 2Beriou G, Moreau A, Cuturi MC. Tolerogenic dendritic cells : applications for solid organ transplantation [ J ]. Curt Opin Organ Transplant, 2012, 17 (1) : 42- 47.
  • 3马麟麟.免疫抑制剂对树突状细胞的影响[J].器官移植,2013,4(6):370-372. 被引量：3
  • 4Zheng DH, Dou LP, Wei YX, et al. Uptake of donor lymphocytes treated with 8-methoxypsoralen and ultraviolet A light by recipient dendritic cells induces CD4 ^+ CD25^+ Foxp3^+ regulatory T cells and down-regulales cardiac allograft rejection [ J]. Biochem Biophys Res Commun, 2010, 395 (4): 540-546.
  • 5魏玉香,周文强,肖漓,郑德华,齐帜,蔡明,钱叶勇,于涛,廖珂,石炳毅.调节性树突细胞对皮肤移植小鼠IL-10＋调节性B细胞的影响[J].解放军医学杂志,2013,38(4):274-278. 被引量：10
  • 6Wojciechowski W, Harris DP, Sprague F, et al. Cytokine- producing effector B cells regulate type 2 immunity to H.polygyrusIJ]. Immunity, 2009, 30 (3): 421-433.
  • 7Inaba K, Inaba M, Romani N, et al. Generation of large numbers of dendritic cells from mouse bone marrow euhures supplemented with granuloeyte/maerophage colony-stimulating factor [J]. J Exp Med, 1992, 176 (6) : 1693-1702.
  • 8石炳毅.调节性树突状细胞在移植免疫研究中的历史沿革[J].中华器官移植杂志,2012,33(6):321-322. 被引量：1
  • 9Sattler S, Ling GS, Xu D, et al. IL-10-produeing regulatory B cells induced by IL-33 ( Breg ( IL-33 ) ) effeetively attenuate mueosal inflammatory responses in the gut[J]. J Autoimmun, 2014, 50. 107-122.

二级参考文献46

  • 1Larsen CP. Ritchie SC. Hendrix R, et al. Regulatkm of immunostimulatory function and costimulatory molecule (B7-I and B7-2) expression on nmrine dendritic cells. J Immunol, 1994. 152(11 ):5208-5219.
  • 2Heath WR, Carbone FIR. Cross presentatkm, dendritic cells, tolerance and inmmnit y. Annu Rev hnmunol. 2(}11, 19 : 47-64.
  • 3Kuwana M. Induction of anergic and regulatory T cells by plasmacytoid dendritic cells and other dendritic cell subsets. Hum Immunol, 2002, 63(12) : 1156-1163.
  • 4Turnquist HR. Thomson AW. Taming the lions: manipulating dendritic cells for use as negative cellular vaccines in organ transplantalion. Curr ()pin Organ Transplant, 20118, 13(4):350-357.
  • 5Chang CC. Ciubotariu R, Manavalan JS. et al. Tolerization of dendritic cells by T (S) cells= the crucial role of inhibitory receptors II.T3 and ILT4. Nal lmmunol. 2002, 3(3):237 -243.
  • 6Zhu I., Wei (;, Jacqueline A, et al. Kidney allograft Iolerance induced by soluble CD83 monotherapy associated with prevalence of tolerogenic dendritic cells and expression of indoleamine 2,3-dioxygenase//ATC 2011). San Diego, 211111. ,m Diego: American Society of "I'ransplantation and the American eiety of Transplant Surgeons, Am J Transplant, 2010: 3G4.
  • 7/klbert ML, Jegathesan M, l)arnell RB. Dendritic cell naturation is required for the cross tolerization of CI)8 T :ells. Nat Immunol, 2001, 2(11):1010-11117.
  • 8Zhang ZX, Yang L, Young KJ, et al. Identification of a previously unknown antigen-specific regulatory T cell and its mechanism of suppression. Nat Med, 200, 6(7) :782-789.
  • 9Zhang I., Thomson AW. New parlners for tolerogenic dendritic cells. Am J Transplant, 2011, 11 (10) :2003-2004.
  • 10Hill M, Thebault P, Segovia M. et al. Cell therapy with autologous tolerogenic dendritic cells induces allograft tolerance through interferon gamma and epstein harr virus induced gene 3. Am J Transplant, 2011, 11 (10) :2036- 2045.

共引文献11

同被引文献5

引证文献1

二级引证文献1

器官移植
2014年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部