气相色谱法检测增香大米中的2-乙酰基吡啶

Determination of 2-acetyl-pyridine in scented rice by gas chromatography

摘要：为了加强对大米生产和销售的监管，有效打击大米造假的不法行为，建立了快速检测增香大米中2-乙酰基吡啶的气相色谱方法。样品采用1．5mol／L盐酸溶液提取，提取液经无水乙醚萃取后进样分析。色谱条件为：进样量2μl，柱温140℃，检测器温度300℃，流速1．5ml／min。该方法条件下，2-乙酰基吡啶在20～200ug／ml范围内线性关系良好，检出限为10mg／kg，定量限为20mg／kg。回收率达到85．08％～96．33％。

In order to strengthen the supervision of rice production and sales, and effectively crack down on the illegal acts of fake rice,the determination method of 2-acetyl-pyridine in scented rice by gas chromatography was developed. The samples were extracted with 1.5 mol/L HCl, then the extracted solution was extracted with absolute ether. The chromatographic parameters were as follows： injection amount of 2 μl, column temperature of 140℃, detector temperature of 300℃, flow rate of 1.5 ml/min. The results showed that： calibration graphs had good linear relationship when 2-acetyl-pyridine amount was in the range of 20;200 μg/ml. The limit of detection was 10 mg/kg and limit of quantitation was 20 mg/kg. The recovery rate was between 85.08%-96.33%.