艾灸对大鼠创伤皮肤组织血管内皮细胞和血管内皮生长因子表达的影响

Effect of moxibustion on vascular endothelial cell and expression of vascular endothelial growth factor in rats with cutaneous wound

目的:探讨艾灸干预大鼠皮肤创伤愈合的作用及作用机制。方法:将24只SD大鼠在其背部制造全层皮肤切线性创伤,以随机数字表法,随机分成治疗组和模型组,每组12只。再根据治疗时间的不同将每组随机分为1d组、3d组、7d组3个时间点小组,每个时间点小组4只大鼠。治疗组取创面局部进行艾灸治疗,每次30min,每日1次。以HE染色法检测修复组织内毛细血管生长情况、血管内皮细胞数;以免疫组化法检测修复组织内血管内皮细胞生长因子的表达情况。结果:大鼠创面愈合指数,治疗7d组在治疗第4天与第8天时均高于模型7d组(均P<0.05)。大鼠创面修复组织毛细血管数,治疗1d组和3d组均高于相应模型组(均P<0.05);治疗7d组毛细血管数低于模型7d组(P<0.05)。大鼠创面修复组织血管内皮细胞数,治疗3d组高于模型3d组(P<0.05);治疗7d组低于模型7d组(P<0.05);治疗1d组与模型1d组比较差异无统计学意义(P>0.05)。大鼠创面修复组织VEGF表达阳性细胞累计积分,治疗3d组高于模型3d组(P<0.05);治疗7d组低于模型7d组(P<0.05);治疗1d组与模型1d组比较差异无统计学意义(P>0.05)。结论:艾灸能促进大鼠皮肤创伤愈合,其机制可能与调节不同时间内创面组织中血管内皮细胞和血管内皮生长因子含量有关。

Objective To explore the effect and action mechanism of moxibustion on healing of cutaneous wound in rats.Methods Twenty-four SD rats were selected and made into linear full-thickness skin injury model.With randomized digital table,rats were randomly divided into a treatment group and a model group,12cases in each one.Then according to treatment time,each group was again divided into a 1dgroup,a 3dgroup and a 7d group,4cases in each one.The moxibustion at injured skin was applied in the treatment group,30min per time,once a day.Hematoxylineosin（HE）staining method was adapted to measure growth status of capillary and number of vascular endothelial cell;immunohistochemical method was used to measure the expression of vascular endothelial growth factor（VEGF）.Results The wound healing indices in the treatment 7dgroup were higher than those in the model 7dgroup on both the 4th day and 8th day after treatment（both P〈0.05）.The number of capillary in the treatment 1dgroup and 3dgroup was higher than that in the model 1dand 3dgroups（both P〈0.05）.The number of capillary in the treatment 7dgroup was lower than that in the model 7dgroup（P〈0.05）.The number of vascular endothelial cell in the treatment 3dgroup was higher than that in the model 3dgroup（P〈0.05）.The number of vascular endothelial cell in the treatment 7dgroup was lower than that in the model 7d group（P〈0.05）.The difference of number of vascular endothelial cell between the treatment 1dgroup and model1dgroup was not significant（P〈0.05）.Positive cells accumulated score of VEGF expression in the treatment 3d group was higher than that in the model 3dgroup（P〈0.05）.Positive cells accumulated score of VEGF expression in the treatment 7dgroup was lower than that in the model 7dgroup（P〈0.05）.The difference of positive cells accumulated score of VEGF expression between the treatment 1dgroup and model 1dgroup was not significant（P〈0.05）.Conclusion Moxibustion could improve the healing of skin wound in rats,which could be related with regulating vascular endothelial cell and VEGF in wound tissue at different time.