摘要
运用聚丙烯酰胺凝胶电泳、高效液相色谱、紫外光谱、红外光谱和核磁共振波谱等手段对不同1,6-酐衍生度的依诺肝素钠的细微结构进行了比较,并进一步测定了它们的抗凝血活性。结果表明,虽然不同1,6-酐衍生度（20.0%~39.7%）依诺肝素钠在结构特征方面相似,但它们的抗Xa和抗IIa因子活性却随着1,6-酐衍生度的增大呈现降低趋势,尤其抗Xa因子活性受1,6-酐衍生度的变化较明显。
The fine structure of enoxaparin sodium samples with different degree of 1, 6-anhydro derivatives were analyzed with polyacrylamide gel electrophoresis, high performance liquid chromatography, ultraviolet spectroscopy, infrared spectroscopy and nuclear magnetic resonance spectroscopy. A further study of anticoagulation activity of enoxaparins was performed, including those on their inhibition activities of coagulation factor Xa（FXa） and thrombin（FIIa）. The results showed that the anti-FXa and-FIIa activities of enoxaparins with different degree of 1, 6-anhydro derivatives（20.0%-39.7%） with similar structure characteristics, had decreasing tendency when the degree of 1, 6-anhydro derivatives increased. Especially, the anti-FXa activity was sensitive to the change of the degree of 1, 6-anhydro derivatives.
出处
《药学学报》
CAS
CSCD
北大核心
2014年第7期1049-1053,共5页
Acta Pharmaceutica Sinica
基金
国家科技支撑计划(2013BAB01B02)
国家自然科学基金资助项目(31070724)
关键词
依诺肝素钠
1
6-酐衍生物
聚丙烯酰胺凝胶电泳
高效液相色谱
抗凝血活性
enoxaparin sodium
1
6-anhydro derivative
polyacrylamide gel electrophoresis
high performance liquid chromatography
anticoagulation activity
