健脾活血袪湿方对肝硬化腹水大鼠肝线粒体的影响

Effects of Jianpi Huoxue Qushi recipe on the hepatic mitochondrial of cirrhotic rats complicated with ascites

目的:观察健脾活血祛湿方对肝硬化腹水大鼠肝线粒体细胞色素C氧化酶活性、ATP含量、ATP酶活性表达的影响。方法:将健康SPF级雄性Wistar大鼠90只,随机分为空白组、模型组、健脾活血祛湿方高、中、低剂量组及秋水仙碱组,运用改良CCL4-酒精综合法造肝硬化腹水大鼠模型。提取肝线粒体进行细胞色素C氧化酶活性、ATP含量、ATP酶活性测定。结果:模型组肝线粒体细胞色素C氧化酶活性、ATP含量、ATP酶活性均明显低于空白组,与空白组相比有显著差异(P<0.01),经过干预后健脾活血袪湿方能升高肝线粒体细胞色素C氧化酶活性、ATP含量、ATP酶活性,其升高程度与中药使用剂量有关,其中健脾活血袪湿方低剂量组与模型组相比差异有统计学意义(P<0.05);健脾活血袪湿方中剂量组及高剂量组与模型组相比具有显著差异(P<0.01);细胞色素C氧化酶活性检测中,秋水仙碱组与模型组比较差异有统计学意义(P<0.05);ATP含量、ATP酶活性检测中,秋水仙碱组与模型组比较差异无统计学意义(P>0.05)。结论:健脾活血袪湿方能引起肝线粒体细胞色素C氧化酶活性、ATP含量、ATP酶活性的表达升高,缓解肝线粒体损伤,改善肝线粒体能量代谢作用,维持肝细胞功能。

Objective： The study aims at observing whether Jianpi Huoxue Qushi recipe can improve the expression of the cytochrome C oxidase activity, ATP content, ATPase activity of liver to cirrhotic rats complicated with ascites treated by TCM. Methods：The animals experiment consists of ninety healthy Wistar rats which were specific pathogen free （SPF）. The rats were divided randomly into the blank group, the model group and the group treating with Jianpi Huoxue Qushi recipe with high, medium and low dosages and the group treating with colchicines. Make the model group with an improved CCL4 combined with alcohol method. Then check their livers cytochrome C oxidase activity, ATP content, ATPase activity. Results： The expression of cytochrome C oxidase activity, ATP content, ATPase activity of blank group has remarkable difference with the model group （P〈0.01）. The marks of cytochrome C oxidase activity, ATP content, ATPase activity expression rise tremendously after treated by Jianpi Huoxue Qushi recipe, the effect among them is related to the dosage use of the TCM. There are significant differences between low dosage group and model group （P〈0.05）, and remarkable difference between high, medium dosage group and model group （P〈0.01）. In the result of cytochrome C oxidase activity, there are significant difference between colchicines group and model group （P〈0.05）. Minor difference exists between colchicines group and the model group in the result of ATP content and ATPase activity （P〉0.05）. Conclusions： Jianpi Huoxue Qushi recipe can increase the expression of cytochrome C oxidase activity, ATP content and ATPase activity. It can increase the energy synthesis of mitochondria and improve the disorder of energy metabolism of mitochondria.