银杏叶总黄酮对大鼠心肌缺血再灌注损伤的保护作用

Protection effects of Yinxing leaf total flavonoid on treating myocardial ischemia-reperfusion injury

目的:探讨银杏叶总黄酮对大鼠心肌缺血再灌注损伤的保护作用及其机制。方法:取成熟健康雄性大鼠50只,随机分为即假手术组、缺血再灌注组、银杏叶总黄酮高剂量组(200mg·kg-1)、中剂量组(100mg·kg-1)、低剂量组(50mg·kg-1)。银杏叶总黄酮组分别于术前灌胃给药,1次/d。连续7d。假手术组和模型组于同一时间灌等量的0.9%氯化钠注射液。采用在体阻断大鼠左冠状动脉30min恢复灌注180min制备心肌缺血再灌注损伤模型。测定心肌梗死面积;测定血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-Px)的活性及丙二醛(MDA)、谷胱甘肽(GSH)含量。结果:与模型组比较,银杏叶总黄酮组缩小心肌梗死面积,同时使血清中丙二醛含量减少,超氧化物歧化酶和谷胱甘肽过氧化酶的活性增加及谷胱甘肽含量增加。而且保护作用随着剂量的增加更加明显。结论:银杏叶总黄酮对大鼠缺血再灌注的心肌能够减少心肌梗死面积,具有不同程度的保护作用,其机制可能与抗氧化作用有关。

Objective： To discuss protection effects of Yinxing leaf total flavonoid on treating myocardial ischemia-reperfusion injury. Methods：50 rats were randomly divided into 5 groups. The Yinxing leaf total flavonoid group was given gavage for 7 days. The blank group and the model group were given 0.9% sodium chloride injection. Myocardial infarction size, serum superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） activity and malondialdehyde （MDA）, glutathione （GSH） content were compared. Results：The Yinxing leaf total flavonoid group has less myocardial infarction size and serum levels of malondialdehyde, more superoxide dismutase and glutathione peroxidase and glutathione content than the model group. Conclusion：The Yinxing leaf total flavonoid can reduce myocardial infarction size, shows protective effects, the mechanism may be related with the antioxidant.