miRNA-135a在阿尔茨海默病患者血液标本及PC-12细胞乏外吐小体中的变化及临床意义

Changes and Clinical Significance of miRNA-135a in Blood Samples and Exosome of PC-12 Cells from Patients with Alzheimer's Disease

目的探讨miRNA-135a在阿尔茨海默病患者血液标本及PC-12细胞乏外吐小体中的变化及临床意义。方法选取轻度认知障碍患者(MCI)9例,痴呆期阿尔茨海默病(DAT)患者21例,健康对照者30例,检测受试者血清、血浆及培养基中miRNA-135a表达水平。结果在乏外吐小体培养基中miRNA-135a相对表达量低于普通培养基(P<0.05);健康对照组血浆中miRNA-135a相对表达量高于血清,也高于乏外吐小体培养基(P<0.05);MCI组、DAT组血清、血浆中miRNA-135a相对表达量均低于对照组(P<0.05)。结论认知障碍患者血miRNA-135a表达量低,检测miRNA-135a对临床诊断阿尔茨海默病有一定价值。

Objective To study the changes and clinical significance of miRNA-135a in blood samples and exosome of PC-12 cells from patients with Alzheimer′s disease.Methods Nine patients with mild cognitive impairment （MCI）,21 patients with dementia of Alzheimer type （ DAT ） and 30 healthy controls were detected for the expression level of miRNA-135a in serum,plasma and medium.Results The relative expression level of miRNA-135a in exosome medium was lower than that in ordinary medium（P〈0.05）.In the healthy control group,the relative expression level of miRNA-135a in plasma was higher than that in serum ,which was also higher than that in exosome medium （P〈0.05）.The relative expression level of serum or plasma miRNA-135a in the MCI group or DAT group was lower than that in the control group （P〈0.05）.Conclusion The expression level of miRNA-135a is low in patients with cognitive impairment ,the detection of miRNA-135 a is valuable in the clinical diagnosis of Alzheimer′s disease .