维生素D3对1～4周龄青农灰鹅血清生化指标、免疫性能及维生素D受体mRNA表达量的影响

Effects of Vitamin D_3 on Serum Biochemical Parameters,Immune Function and Vitamin D Receptor mRNA Expression Level of Qingnonghui Geese Aged from 1 to 4 Weeks

本试验旨在研究维生素D3对1～4周龄青农灰鹅血清生化指标、免疫性能及维生素D受体（VDR）mRNA表达量的影响,以确定鹅饲粮中维生素D3的适宜添加水平.选择1日龄青农灰鹅360只,随机分为6个组,每组6个重复,每个重复10只鹅.Ⅰ组为对照组,饲喂玉米-豆粕型基础饲粮;Ⅱ～Ⅵ组分别在基础饲粮中添加200、400、800、1 600、3 200 IU/kg维生素D3.试验期为4周.结果表明：1）Ⅴ、Ⅵ组血清钙、磷、维生素D3含量显著高于Ⅰ组（P＜0.05）,Ⅱ～Ⅵ组血清碱性磷酸酶活性显著低于Ⅰ组（P＜0.05）,各组血清钙结合蛋白、骨钙素、降钙素、甲状旁腺素含量无显著差异（P＞0.05）.2）Ⅲ组胸腺指数显著高于其他各组（P＜0.05）,各组脾脏指数、法氏囊指数及血清溶菌酶活性无显著差异（P＞0.05）.3）Ⅳ组免疫前和免疫后7d禽流感抗体效价显著高于Ⅰ组（P＜0.05）,VDR mRNA表达量显著或极显著高于其他各组（P＜0.05或P＜0.01）.由此可见,饲粮添加200～800 IU/kg维生素D3能提高1～4周龄鹅血清生化指标、免疫性能和VDR mRNA表达量.

This experiment was conducted to study the effects of vitamin D3 on serum biochemical parameters,immune function and vitamin D receptor （VDR） mRNA expression level of Qingnonghui geese aged from 1 to 4 weeks,and to find the appropriate supplemental level of vitamin D3 in diets of geese.A total of 360 Qingnonghui geese （one-day-old） were randomly divided into 6 groups with 6 replicates per group and 10 geese per replicate.Geese in group Ⅰ （control group） were fed a corn-soybean meal basal diet,and geese in groups Ⅱ to Ⅵ were fed the basal diets supplemented with 200,400,800,1 600 and 3 200 IU/kg vitamin D3,respectively.The experiment lasted for 4 weeks.The results showed as follows：1） the contents of calcium,phosphorus and vitamin D3 in serum in groups Ⅴ and Ⅵ were significantly higher than those in group Ⅰ （P＜0.05）,while there were no significant effects on the contents of calcium binding protein,bone gla protein,calcitonin and parathormone in serum among all groups （P＞0.05）.2） The hymus index in group Ⅲ was significantly higher than that in other groups （P＜0.05）,while there were no significant effects on the spleen index,bursa of Fabricius index and serum lysozyme activity among all groups （P＞0.05）.3） The bird flu antibody titer at pre-immune and immune after 7 days in group Ⅳ were significantly higher than that in group Ⅰ （P＜0.05）,and the VDR mRNA expression level in group Ⅳ were significantly higher than that in other groups （P＜0.05 or P＜0.01）.In conclusion,dietary supplemented with 200 to 800 IU/kg vitamin D3 can increase the serum biochemical parameters,immune function and VDR mRNA expression level of geese aged from 1 to 4 weeks.